{"id":{"repo_id":"central-wash","oai_identifier":"oai:digitalcommons.cwu.edu:etd-1239"},"canonical_url":"https://search.dev.ndltd.org/etd/central-wash/oai:digitalcommons.cwu.edu:etd-1239","repository":{"repo_id":"central-wash","name":"Central Washington University","base_url":"https://digitalcommons.cwu.edu/do/oai/"},"display":{"title":"The Evolution of Sex Determination and the DMRT1 Gene in the Japanese Gecko (Gekko japonicus)","abstract":"There are different sex-determining mechanisms in our environment, which are separated into two groups known as genotypic sex determination (GSD) and environmental sex determination. The most well-known mechanism in the ESD group is temperature-dependent sex determination (TSD). In this study, the presence of the Doublesex and mab-3-related Transcription Factor (<em>Dmrt1</em>) gene was observed during embryonic development in geckos with a TSD mechanism. To do this, I observed the rate of transcription of the <em>Dmrt1</em> gene in the Gecko species <em>Gekko japonicus</em>. Pregnant geckos were caught around Nanjing, China. Once the females laid their eggs, the eggs were then randomly placed in one of three different temperature regimes (24°C, 28°C, 32°C). The embryos, the main target, were dissected at two points during development and total RNA was extracted. A relative rate of transcription of the <em>Dmrt1</em> gene was assessed using quantitative PCR (qPCR). I was not able to quantify the use of the <em>Dmrt1</em> gene between males and females. However, my study does not allow me to exclude the possibility that <em>G. japonicus</em> may use a GSD system with a TSD override, instead of the earlier proposed TSD system.","abstract_html":"There are different sex-determining mechanisms in our environment, which are separated into two groups known as genotypic sex determination (GSD) and environmental sex determination. The most well-known mechanism in the ESD group is temperature-dependent sex determination (TSD). In this study, the presence of the Doublesex and mab-3-related Transcription Factor (&lt;em&gt;Dmrt1&lt;/em&gt;) gene was observed during embryonic development in geckos with a TSD mechanism. To do this, I observed the rate of transcription of the &lt;em&gt;Dmrt1&lt;/em&gt; gene in the Gecko species &lt;em&gt;Gekko japonicus&lt;/em&gt;. Pregnant geckos were caught around Nanjing, China. Once the females laid their eggs, the eggs were then randomly placed in one of three different temperature regimes (24°C, 28°C, 32°C). The embryos, the main target, were dissected at two points during development and total RNA was extracted. A relative rate of transcription of the &lt;em&gt;Dmrt1&lt;/em&gt; gene was assessed using quantitative PCR (qPCR). I was not able to quantify the use of the &lt;em&gt;Dmrt1&lt;/em&gt; gene between males and females. However, my study does not allow me to exclude the possibility that &lt;em&gt;G. japonicus&lt;/em&gt; may use a GSD system with a TSD override, instead of the earlier proposed TSD system.","abstract_has_math":false,"creators":["Mullen, Lisa-Marie"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":null,"degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Lixing Sun","April K. Binder","Joseph Lorenz"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-01-01T08:00:00Z","date_published":"2015-01-01T08:00:00Z","updated_at":"2026-07-24T01:36:42Z","subjects":["Sex Determination","DMRT1","reptile","TSD","Ecology and Evolutionary Biology","Evolution","Genetics"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.cwu.edu/etd/229","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lixing Sun","April K. 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The most well-known mechanism in the ESD group is temperature-dependent sex determination (TSD). In this study, the presence of the Doublesex and mab-3-related Transcription Factor (<em>Dmrt1</em>) gene was observed during embryonic development in geckos with a TSD mechanism. To do this, I observed the rate of transcription of the <em>Dmrt1</em> gene in the Gecko species <em>Gekko japonicus</em>. Pregnant geckos were caught around Nanjing, China. Once the females laid their eggs, the eggs were then randomly placed in one of three different temperature regimes (24°C, 28°C, 32°C). The embryos, the main target, were dissected at two points during development and total RNA was extracted. A relative rate of transcription of the <em>Dmrt1</em> gene was assessed using quantitative PCR (qPCR). I was not able to quantify the use of the <em>Dmrt1</em> gene between males and females. However, my study does not allow me to exclude the possibility that <em>G. japonicus</em> may use a GSD system with a TSD override, instead of the earlier proposed TSD system."]},{"key":"dc:title","label":"Title","values":["The Evolution of Sex Determination and the DMRT1 Gene in the Japanese Gecko (Gekko japonicus)"]}]}],"canonical_facts":{"dc:contributor":["Lixing Sun","April K. Binder","Joseph Lorenz"],"dc:creator":["Mullen, Lisa-Marie"],"dc:date.available":["2016-09-09T07:00:00Z"],"dc:description.abstract":["There are different sex-determining mechanisms in our environment, which are separated into two groups known as genotypic sex determination (GSD) and environmental sex determination. The most well-known mechanism in the ESD group is temperature-dependent sex determination (TSD). In this study, the presence of the Doublesex and mab-3-related Transcription Factor (<em>Dmrt1</em>) gene was observed during embryonic development in geckos with a TSD mechanism. To do this, I observed the rate of transcription of the <em>Dmrt1</em> gene in the Gecko species <em>Gekko japonicus</em>. Pregnant geckos were caught around Nanjing, China. Once the females laid their eggs, the eggs were then randomly placed in one of three different temperature regimes (24°C, 28°C, 32°C). The embryos, the main target, were dissected at two points during development and total RNA was extracted. A relative rate of transcription of the <em>Dmrt1</em> gene was assessed using quantitative PCR (qPCR). I was not able to quantify the use of the <em>Dmrt1</em> gene between males and females. However, my study does not allow me to exclude the possibility that <em>G. japonicus</em> may use a GSD system with a TSD override, instead of the earlier proposed TSD system."],"dc:identifier":["https://digitalcommons.cwu.edu/etd/229"],"dc:language":["English"],"dc:subject":["Sex Determination","DMRT1","reptile","TSD","Ecology and Evolutionary Biology","Evolution","Genetics"],"dc:title":["The Evolution of Sex Determination and the DMRT1 Gene in the Japanese Gecko (Gekko japonicus)"],"dc:type":["Text"],"thesis:degree_discipline":["Biology"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T01:36:42Z"}