{"id":{"repo_id":"cent-lancashire","oai_identifier":"oai:clok.uclan.ac.uk:9563"},"canonical_url":"https://search.dev.ndltd.org/etd/cent-lancashire/oai:clok.uclan.ac.uk:9563","repository":{"repo_id":"cent-lancashire","name":"University of Central Lancashire","base_url":"https://clok.uclan.ac.uk/cgi/oai2"},"display":{"title":"The role of Calcineurin in skeletal muscle differentiation","abstract":"1. Strong evidence suggests that Calcineurin levels are higher in fast muscle fibers compared to slow-twitch in resting skeletal muscles. Activation of the Calcineurin in 4 skeletal muscle myocytes selectively up-regulates slowfiber- specific gene promoters through a mechanism involving the transcription factor NFATcI. The Calcineurin pathway itself was down-regulated when rat skeletal muscles were chronically stimulated at 10 FIz for a period of 3 weeks illustrating adaptation. 2. Skeletal muscles that received chronic stimulation treatment showed a significant increase in mitochondrial content. Histochemical studies detected a change towards the slow phenotype, through the decrease of fast-twitch Type Bib fiber content in fast skeletal muscles. Metabolic activity was not significantly affected through this period of chronic stimulation. 3. Cyclosporin A was not able to prevent this initial transition towards the slow phenotype, even though 3 weeks of 10 Hz chronic stimulation was insufficient to cause marked changes in the skeletal muscle metabolism. This suggests an incomplete fast-to-slow transformation was elicited by these conditions. 4. Stimulation of L6 myocytes with the calcium ionophore 4-Bromo-A23 187 (I 0 M) a partial fast-to-slow transformation occurred. It is likely that this change was brought about by a number of processes including NFAT translocation to the nucleus.","abstract_html":"1. Strong evidence suggests that Calcineurin levels are higher in fast muscle fibers compared to slow-twitch in resting skeletal muscles. Activation of the Calcineurin in 4 skeletal muscle myocytes selectively up-regulates slowfiber- specific gene promoters through a mechanism involving the transcription factor NFATcI. The Calcineurin pathway itself was down-regulated when rat skeletal muscles were chronically stimulated at 10 FIz for a period of 3 weeks illustrating adaptation. 2. Skeletal muscles that received chronic stimulation treatment showed a significant increase in mitochondrial content. Histochemical studies detected a change towards the slow phenotype, through the decrease of fast-twitch Type Bib fiber content in fast skeletal muscles. Metabolic activity was not significantly affected through this period of chronic stimulation. 3. Cyclosporin A was not able to prevent this initial transition towards the slow phenotype, even though 3 weeks of 10 Hz chronic stimulation was insufficient to cause marked changes in the skeletal muscle metabolism. This suggests an incomplete fast-to-slow transformation was elicited by these conditions. 4. Stimulation of L6 myocytes with the calcium ionophore 4-Bromo-A23 187 (I 0 M) a partial fast-to-slow transformation occurred. It is likely that this change was brought about by a number of processes including NFAT translocation to the nucleus.","abstract_has_math":false,"creators":["Manolopoulus, Panagiotis"],"institution":"University of Central Lancashire","degree_name":"msc","degree_level":"masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002-10","date_published":"2002-10","updated_at":"2026-07-24T01:35:46Z","subjects":["B230 - Pharmacy"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Manolopoulus, Panagiotis"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2002-10"]},{"key":"dc:date.issued","label":"Date","values":["2002-10"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Biological Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Central Lancashire"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://knowledge.lancashire.ac.uk/id/eprint/9563/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["msc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["B230 - Pharmacy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://knowledge.lancashire.ac.uk/id/eprint/9563/1/Panagiotis%20Manolopoulos%20Oct02%20The%20role%20of%20Calcineurin%20in%20skeletal%20muscle%20differentiation%20Degree%20of%20Masters%20of%20Science%20unpublished%20Oct02%20University%20of%20Central%20Lancashire%20Biological%20Sciences%20161.pdf","https://knowledge.lancashire.ac.uk/id/eprint/9563/7/Panagiotis%20Manolopoulos.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["1. Strong evidence suggests that Calcineurin levels are higher in fast muscle fibers compared to slow-twitch in resting skeletal muscles. Activation of the Calcineurin in 4 skeletal muscle myocytes selectively up-regulates slowfiber- specific gene promoters through a mechanism involving the transcription factor NFATcI. The Calcineurin pathway itself was down-regulated when rat skeletal muscles were chronically stimulated at 10 FIz for a period of 3 weeks illustrating adaptation. 2. Skeletal muscles that received chronic stimulation treatment showed a significant increase in mitochondrial content. Histochemical studies detected a change towards the slow phenotype, through the decrease of fast-twitch Type Bib fiber content in fast skeletal muscles. Metabolic activity was not significantly affected through this period of chronic stimulation. 3. Cyclosporin A was not able to prevent this initial transition towards the slow phenotype, even though 3 weeks of 10 Hz chronic stimulation was insufficient to cause marked changes in the skeletal muscle metabolism. This suggests an incomplete fast-to-slow transformation was elicited by these conditions. 4. Stimulation of L6 myocytes with the calcium ionophore 4-Bromo-A23 187 (I 0 M) a partial fast-to-slow transformation occurred. It is likely that this change was brought about by a number of processes including NFAT translocation to the nucleus."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The role of Calcineurin in skeletal muscle differentiation"]}]}],"canonical_facts":{"dc:creator":["Manolopoulus, Panagiotis"],"dc:date":["2002-10"],"dc:date.issued":["2002-10"],"dc:description.abstract":["1. Strong evidence suggests that Calcineurin levels are higher in fast muscle fibers compared to slow-twitch in resting skeletal muscles. Activation of the Calcineurin in 4 skeletal muscle myocytes selectively up-regulates slowfiber- specific gene promoters through a mechanism involving the transcription factor NFATcI. The Calcineurin pathway itself was down-regulated when rat skeletal muscles were chronically stimulated at 10 FIz for a period of 3 weeks illustrating adaptation. 2. Skeletal muscles that received chronic stimulation treatment showed a significant increase in mitochondrial content. Histochemical studies detected a change towards the slow phenotype, through the decrease of fast-twitch Type Bib fiber content in fast skeletal muscles. Metabolic activity was not significantly affected through this period of chronic stimulation. 3. Cyclosporin A was not able to prevent this initial transition towards the slow phenotype, even though 3 weeks of 10 Hz chronic stimulation was insufficient to cause marked changes in the skeletal muscle metabolism. This suggests an incomplete fast-to-slow transformation was elicited by these conditions. 4. Stimulation of L6 myocytes with the calcium ionophore 4-Bromo-A23 187 (I 0 M) a partial fast-to-slow transformation occurred. It is likely that this change was brought about by a number of processes including NFAT translocation to the nucleus."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://knowledge.lancashire.ac.uk/id/eprint/9563/1/Panagiotis%20Manolopoulos%20Oct02%20The%20role%20of%20Calcineurin%20in%20skeletal%20muscle%20differentiation%20Degree%20of%20Masters%20of%20Science%20unpublished%20Oct02%20University%20of%20Central%20Lancashire%20Biological%20Sciences%20161.pdf","https://knowledge.lancashire.ac.uk/id/eprint/9563/7/Panagiotis%20Manolopoulos.pdf"],"dc:language":["en"],"dc:publisher.department":["Biological Sciences"],"dc:publisher.institution":["University of Central Lancashire"],"dc:relation.isreferencedby":["https://knowledge.lancashire.ac.uk/id/eprint/9563/"],"dc:subject":["B230 - Pharmacy"],"dc:title":["The role of Calcineurin in skeletal muscle differentiation"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"],"dc:type.qualificationname":["msc"]},"updated_at":"2026-07-24T01:35:46Z"}