Influence of magnesium on salivary gland secretion: physiological and pathophysiological studies
type: CreativeWork
description: The divalent abundant cation Magnesium (Mg2+) has been known to play an important regulatory role in the stimulus-secretion coupling events in a number of epithelial secretory cells including the exocrine pancreas, lachrymal and the parietal cells. Since the salivary glands acinar cells have a similar structure and function as the exocrine pancreas, the stomach and the lachrymal gland, it was decided to investigate specifically the effect of perturbation of extracellular magnesium ([Mg2+]0) on both basal and secretagogue-evoked amylase secretion and total protein output and intracellular free calcium concentrations ([Ca2+]i) in the rat submandibular and parotid glands using spectrofluorimetry and spectroscopic techniques. The results have shown that both zero and elevated (5 and 10 mM) [Mg2+]0 can significantly (P<0.01) inhibit basal amylase or protein output compared to normal (1.1 mM) [Mg2+]0 Either acetylcholine (ACh), noradrenaline (NA) or Phenylephrine (Phe) can elicit marked increases in amylase secretion and total protein output from the isolated parotid and submandibular gland segments respectively in normal [Mg2+]0. In contrast, in the presence of either zero or elevated (5 and 10 mM) [Mg2+]0 the ACh, NA and Phe-evoked amylase secretion and total protein output were markedly attenuated when compared to normal (1.1 mM) Mg2+]0. The inhibitory effect of zero [Mg2+]0 was much more pronounced when compared to 10 mM [Mg2+]0. A perturbation of [Mg2+]0 had little or no effect on the Electrical Field Stimulation (EFS) -evoked amylase responses in the parotid gland. Isoprenaline-evoked amylase secretion revealed a different pattern of secretion in the presence of [Mg2+]0 perturbation. In Fura-2 loaded salivary acinar cells a perturbation of [Mg2+]0 had no significant effect on basal [Ca2+]i in the parotid gland but elevated (5 and 10 mM) [Mg2+]0 attenuated [Ca2+]i in acinar cells taken from submandibular gland. In acinar cells from both glands ACh (105 M), evoked a m…
abstract: The divalent abundant cation Magnesium (Mg2+) has been known to play an important regulatory role in the stimulus-secretion coupling events in a number of epithelial secretory cells including the exocrine pancreas, lachrymal and the parietal cells. Since the salivary glands acinar cells have a similar structure and function as the exocrine pancreas, the stomach and the lachrymal gland, it was decided to investigate specifically the effect of perturbation of extracellular magnesium ([Mg2+]0) on both basal and secretagogue-evoked amylase secretion and total protein output and intracellular free calcium concentrations ([Ca2+]i) in the rat submandibular and parotid glands using spectrofluorimetry and spectroscopic techniques. The results have shown that both zero and elevated (5 and 10 mM) [Mg2+]0 can significantly (P<0.01) inhibit basal amylase or protein output compared to normal (1.1 mM) [Mg2+]0 Either acetylcholine (ACh), noradrenaline (NA) or Phenylephrine (Phe) can elicit marked increases in amylase secretion and total protein output from the isolated parotid and submandibular gland segments respectively in normal [Mg2+]0. In contrast, in the presence of either zero or elevated (5 and 10 mM) [Mg2+]0 the ACh, NA and Phe-evoked amylase secretion and total protein output were markedly attenuated when compared to normal (1.1 mM) Mg2+]0. The inhibitory effect of zero [Mg2+]0 was much more pronounced when compared to 10 mM [Mg2+]0. A perturbation of [Mg2+]0 had little or no effect on the Electrical Field Stimulation (EFS) -evoked amylase responses in the parotid gland. Isoprenaline-evoked amylase secretion revealed a different pattern of secretion in the presence of [Mg2+]0 perturbation. In Fura-2 loaded salivary acinar cells a perturbation of [Mg2+]0 had no significant effect on basal [Ca2+]i in the parotid gland but elevated (5 and 10 mM) [Mg2+]0 attenuated [Ca2+]i in acinar cells taken from submandibular gland. In acinar cells from both glands ACh (105 M), evoked a m…
author: Mata, Antonio
language: en
keywords: B121 - Clinical physiology
date published: 2003-08
date modified: 2026-07-24T01:35:46Z
provider: University of Central Lancashire
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Central Lancashire