Mechanism of exocrine pancreatic insufficiency during diabetes mellitus
type: CreativeWork
description: The interaction between endocrine and exocrine pancreas has not been properly and thoroughly investigated especially during diabetic conditions. This study was designed to investigate the interaction of the islet hormone insulin with the cholinergic neurotransmitter acetylcholine (ACh) and the gut hormone cholecystokinin-octapeptide (CCK-8), measuring the amylase output, intracellular free calcium concentration ([Ca2i), intracellular free magnesium concentration ([Mg2 ]) and pancreatic juice secretion in the isolated pancreatic segments and the anaesthetised rat preparations of diabetic and age-matched control rats. Alter 6-8 weeks of diabetes induction, diabetic rats in all the experimental groups weighed significantly (pc0.05) less (236.88 ± 7.2 g) compared to age-matched controls (388.68 ± 30.8 g). Similarly, blood glucose levels were significantly (pc0.05) higher (>>500 mg dF') in diabetic rats compared to age-matched controls (92.67 ± 2.41 mg Basal values for pancreatic juice flow, [Ca 2 ']1, and [Mg2 ] in diabetic pancreas were 0.45 ± 0.046 p1 mm 4 , 53.8 ± 11.5 nM, 1.07 ± 0.023 mlvi, respectively. Basal values for pancreatic juice flow, [Ca 2 ]; and [Mg2 ']1 in age-matched controls were 0.65 ± 0.021 p1 min', 76.8 ± 0.85 nM and 1.21 ± 0.026 mM, respectively. Stimulation of isolated pancreatic segments from normal and diabetic rats with different concentrations of either ACh or CCK-8 resulted in marked increases of amylase output. The effects of the secretagogues were less pronounced on the pancreatic segments from diabetic animals compared to age-matched controls. Amylase output in the presence of either 10 5M ACh or 10 8M CCK-8 from control healthy animals was 10.75 ± 0.82 U mI 1 (100 mg oftissue' and 25.6 ± 2.5 U ml' (100 mg oftissue, respectively. The effects of the two secretagogues were less pronounced in pancreatic segments taken from diabetic animals when compared to age-matched control animals. Amylase output in the presence of 10 5M ACh and 10 8M CCK-…
abstract: The interaction between endocrine and exocrine pancreas has not been properly and thoroughly investigated especially during diabetic conditions. This study was designed to investigate the interaction of the islet hormone insulin with the cholinergic neurotransmitter acetylcholine (ACh) and the gut hormone cholecystokinin-octapeptide (CCK-8), measuring the amylase output, intracellular free calcium concentration ([Ca2i), intracellular free magnesium concentration ([Mg2 ]) and pancreatic juice secretion in the isolated pancreatic segments and the anaesthetised rat preparations of diabetic and age-matched control rats. Alter 6-8 weeks of diabetes induction, diabetic rats in all the experimental groups weighed significantly (pc0.05) less (236.88 ± 7.2 g) compared to age-matched controls (388.68 ± 30.8 g). Similarly, blood glucose levels were significantly (pc0.05) higher (>>500 mg dF') in diabetic rats compared to age-matched controls (92.67 ± 2.41 mg Basal values for pancreatic juice flow, [Ca 2 ']1, and [Mg2 ] in diabetic pancreas were 0.45 ± 0.046 p1 mm 4 , 53.8 ± 11.5 nM, 1.07 ± 0.023 mlvi, respectively. Basal values for pancreatic juice flow, [Ca 2 ]; and [Mg2 ']1 in age-matched controls were 0.65 ± 0.021 p1 min', 76.8 ± 0.85 nM and 1.21 ± 0.026 mM, respectively. Stimulation of isolated pancreatic segments from normal and diabetic rats with different concentrations of either ACh or CCK-8 resulted in marked increases of amylase output. The effects of the secretagogues were less pronounced on the pancreatic segments from diabetic animals compared to age-matched controls. Amylase output in the presence of either 10 5M ACh or 10 8M CCK-8 from control healthy animals was 10.75 ± 0.82 U mI 1 (100 mg oftissue' and 25.6 ± 2.5 U ml' (100 mg oftissue, respectively. The effects of the two secretagogues were less pronounced in pancreatic segments taken from diabetic animals when compared to age-matched control animals. Amylase output in the presence of 10 5M ACh and 10 8M CCK-…
author: Kafsafados, Nickolaos
language: en
keywords: F410 - Forensic science
date published: 2004-01
date modified: 2026-07-24T01:36:13Z
provider: University of Central Lancashire
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Central Lancashire