Can curcumin improve the methotrexate based treatment for rheumatoid arthritis?
type: CreativeWork
description: Rheumatoid arthritis (RA) is an autoimmune disorder characterised by its varied and unpredictable origin, eventual destruction of cartilage and bone and the perpetual length of treatment involved. Even though significant developments have taken place over the past couple of decades in the treatment, the efficacy of drugs for RA is a major concern due to the side effects involved. Methotrexate (MTX) is currently used as first line treatment due to its ability to modify the rheumatic conditions so that disease progression can be prevented. However, at the same time prolonged exposure to MTX can lead to severe side effects such as lung fibrosis and hepatotoxicity. Recent research has focused on developing an alternative to MTX with similar efficacy but with reduced adverse effects. One such promising option is curcumin, an active compound extracted from Indian spice Turmeric. Various studies have indicated the synergistic properties exhibited by curcumin and its ability to modulate the underlying inflammatory pathways involved in RA. However, no previous studies have been reported with regards to using a combination of MTX and curcumin for the treatment of RA. A novel RP-HPLC stability indicating method was developed in order to establish the compatibility of the two compounds. The method was developed using Waters Reverse Phase (XBridgeTM Shield RP18 4.6x250 mm, 5 µM) column. A gradient system, consisting of two mobile phases with acetonitrile concentrations of 35% and 60% respectively, was designed to optimise the separation of the two compounds while taking into consideration the difference in hydrophobicity. The wavelengths for detection were 305 nm and 430 nm for MTX and curcumin, respectively. The retention time for MTX and curcumin was 4.8 ± 0.10 min and 12.3 ± 0.10 min, respectively. The Page 4 of 192 total run time of analysis was 25 minutes. The developed method was validated for parameters such as accuracy, precision, linearity, limit of detection and lower …
abstract: Rheumatoid arthritis (RA) is an autoimmune disorder characterised by its varied and unpredictable origin, eventual destruction of cartilage and bone and the perpetual length of treatment involved. Even though significant developments have taken place over the past couple of decades in the treatment, the efficacy of drugs for RA is a major concern due to the side effects involved. Methotrexate (MTX) is currently used as first line treatment due to its ability to modify the rheumatic conditions so that disease progression can be prevented. However, at the same time prolonged exposure to MTX can lead to severe side effects such as lung fibrosis and hepatotoxicity. Recent research has focused on developing an alternative to MTX with similar efficacy but with reduced adverse effects. One such promising option is curcumin, an active compound extracted from Indian spice Turmeric. Various studies have indicated the synergistic properties exhibited by curcumin and its ability to modulate the underlying inflammatory pathways involved in RA. However, no previous studies have been reported with regards to using a combination of MTX and curcumin for the treatment of RA. A novel RP-HPLC stability indicating method was developed in order to establish the compatibility of the two compounds. The method was developed using Waters Reverse Phase (XBridgeTM Shield RP18 4.6x250 mm, 5 µM) column. A gradient system, consisting of two mobile phases with acetonitrile concentrations of 35% and 60% respectively, was designed to optimise the separation of the two compounds while taking into consideration the difference in hydrophobicity. The wavelengths for detection were 305 nm and 430 nm for MTX and curcumin, respectively. The retention time for MTX and curcumin was 4.8 ± 0.10 min and 12.3 ± 0.10 min, respectively. The Page 4 of 192 total run time of analysis was 25 minutes. The developed method was validated for parameters such as accuracy, precision, linearity, limit of detection and lower …
author: Darekar, Ashish Sahadev
language: en
keywords: C130 - Cell biology, C400 - Genetics, C550 - Immunology, C700 - Molecular biology, biophysics & biochemistry
date published: 2016-11
date modified: 2026-07-24T01:36:13Z
provider: University of Central Lancashire
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Central Lancashire