A comparison of different methods of determination of amino acid profiles in mammalian tissues: a study of teratogenic effects of vigabatrin and alcohol
type: CreativeWork
description: Amino acid analysis has been used extensively as a biochemical tool for the diagnosis and monitoring of amino acid levels in normal and diseased conditions resulting from inborn errors of metabolism. Normaly, a volume of 1 ml of venous blood is often recommended but this is a serious problem when blood has to be taken from new born and small children who have low blood volume. Moreover, in the treatment of such disease as epilepsy with anti-epileptic drugs (AED) and in the incidence of foetal alcohol syndrome (FAS), it is of paramOunt importance to monitor the levels of amino acids in both the mother and their babies in order to determine any possible inborn error which may occur as a result of either drug treatment or alcohol intake. One of the primary aims of this study was to develop a new micro-capillary technique employing a small volume of human blood for amino acid analysis using ion-exchange chromatography. Blood was collected from healthy adult subjects and newborn babies and infants of the United Arab Emirates. Three methods for the collection and preparation of the amino acid profile by ion-exchange chromatography were compared; a new micro-capillary method, a standard filter paper technique and the standard venous blood methodl In addition, the project investigated the teratogenic effects of the AED, vigabatrin (VGB) and acute alcohol administration on amino acid metabolism in maternal blood, placenta and embryo of Theiler Outbred (TO) mice. The mice were injected intraperitoneally with either 400 mg kg VOB on gestation day 10 or with 70 mg kj' -150 mg kg' methionine and 0.03 ml kg' absolute alcohol on gestation days 7-12. Following injection, blood samples, placentas and embryos were collected at 3.5, 6.0 and 9.0 hours after treatment for the analysis of amino acids. The results have shown that intra-sample reproducibility in the filter paper and capillary blood from the same individual was less than 20% and 5%, respectively. The greatest variability in…
abstract: Amino acid analysis has been used extensively as a biochemical tool for the diagnosis and monitoring of amino acid levels in normal and diseased conditions resulting from inborn errors of metabolism. Normaly, a volume of 1 ml of venous blood is often recommended but this is a serious problem when blood has to be taken from new born and small children who have low blood volume. Moreover, in the treatment of such disease as epilepsy with anti-epileptic drugs (AED) and in the incidence of foetal alcohol syndrome (FAS), it is of paramOunt importance to monitor the levels of amino acids in both the mother and their babies in order to determine any possible inborn error which may occur as a result of either drug treatment or alcohol intake. One of the primary aims of this study was to develop a new micro-capillary technique employing a small volume of human blood for amino acid analysis using ion-exchange chromatography. Blood was collected from healthy adult subjects and newborn babies and infants of the United Arab Emirates. Three methods for the collection and preparation of the amino acid profile by ion-exchange chromatography were compared; a new micro-capillary method, a standard filter paper technique and the standard venous blood methodl In addition, the project investigated the teratogenic effects of the AED, vigabatrin (VGB) and acute alcohol administration on amino acid metabolism in maternal blood, placenta and embryo of Theiler Outbred (TO) mice. The mice were injected intraperitoneally with either 400 mg kg VOB on gestation day 10 or with 70 mg kj' -150 mg kg' methionine and 0.03 ml kg' absolute alcohol on gestation days 7-12. Following injection, blood samples, placentas and embryos were collected at 3.5, 6.0 and 9.0 hours after treatment for the analysis of amino acids. The results have shown that intra-sample reproducibility in the filter paper and capillary blood from the same individual was less than 20% and 5%, respectively. The greatest variability in…
author: Al Balooshi, Ahmed Ibrahim
language: en
keywords: C990 - Biological sciences not elsewhere classified
date published: 2003
date modified: 2026-07-24T01:36:00Z
provider: University of Central Lancashire
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Central Lancashire