{"id":{"repo_id":"carleton","oai_identifier":"oai:carleton.scholaris.ca:20.500.14718/40407"},"canonical_url":"https://search.dev.ndltd.org/etd/carleton/oai:carleton.scholaris.ca:20.500.14718/40407","repository":{"repo_id":"carleton","name":"Carleton University","base_url":"https://carleton.scholaris.ca/server/oai/request"},"display":{"title":"Conservation of Translation Initiation Between Prokaryotes and Eukaryotes","abstract":"While the fundamental goals of translation initiation are the same for all cells, it is the most phylogenetically diverse step within translation. Until recently, there has been no evidence of a molecular mechanism that can initiate translation in both prokaryotes and eukaryotes. Colussi et al. (2015) reported that the eukaryotic PSIV IGR IRES can initiate prokaryotic translation and effectively circumvent domain-specific divergences. This novel discovery led us to investigate whether this IRES is unique in its ability to initiate prokaryotic translation and attempt to elucidate any distinguishing characteristics that allow for its mechanism of action to occur. Our results indicate that this IRES is unique in its ability to initiate prokaryotic translation. It also appears that the structural integrity of this IRES is less critical in prokaryotes than eukaryotes, and complementary interactions between Domain III and the 16S rRNA may contribute to this IRES' mechanism of action.","abstract_html":"While the fundamental goals of translation initiation are the same for all cells, it is the most phylogenetically diverse step within translation. Until recently, there has been no evidence of a molecular mechanism that can initiate translation in both prokaryotes and eukaryotes. Colussi et al. (2015) reported that the eukaryotic PSIV IGR IRES can initiate prokaryotic translation and effectively circumvent domain-specific divergences. This novel discovery led us to investigate whether this IRES is unique in its ability to initiate prokaryotic translation and attempt to elucidate any distinguishing characteristics that allow for its mechanism of action to occur. Our results indicate that this IRES is unique in its ability to initiate prokaryotic translation. It also appears that the structural integrity of this IRES is less critical in prokaryotes than eukaryotes, and complementary interactions between Domain III and the 16S rRNA may contribute to this IRES' mechanism of action.","abstract_has_math":false,"creators":["Puchacz, Nathalie Martha"],"institution":"Carleton University","degree_name":"Master of Science (M.Sc.)","degree_level":"Master's","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019","date_published":"2019","updated_at":"2026-07-24T01:34:20Z","subjects":[],"languages":["en"],"rights":["Copyright © 2019 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. 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Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. 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This novel discovery led us to investigate whether this IRES is unique in its ability to initiate prokaryotic translation and attempt to elucidate any distinguishing characteristics that allow for its mechanism of action to occur. Our results indicate that this IRES is unique in its ability to initiate prokaryotic translation. It also appears that the structural integrity of this IRES is less critical in prokaryotes than eukaryotes, and complementary interactions between Domain III and the 16S rRNA may contribute to this IRES' mechanism of action."]},{"key":"dc:title","label":"Title","values":["Conservation of Translation Initiation Between Prokaryotes and Eukaryotes"]}]}],"canonical_facts":{"dc:creator":["Puchacz, Nathalie Martha"],"dc:date.accessioned":["2025-04-08T19:59:51Z"],"dc:date.available":["2025-04-08T19:59:51Z"],"dc:date.issued":["2019"],"dc:description.abstract":["While the fundamental goals of translation initiation are the same for all cells, it is the most phylogenetically diverse step within translation. Until recently, there has been no evidence of a molecular mechanism that can initiate translation in both prokaryotes and eukaryotes. Colussi et al. (2015) reported that the eukaryotic PSIV IGR IRES can initiate prokaryotic translation and effectively circumvent domain-specific divergences. This novel discovery led us to investigate whether this IRES is unique in its ability to initiate prokaryotic translation and attempt to elucidate any distinguishing characteristics that allow for its mechanism of action to occur. Our results indicate that this IRES is unique in its ability to initiate prokaryotic translation. It also appears that the structural integrity of this IRES is less critical in prokaryotes than eukaryotes, and complementary interactions between Domain III and the 16S rRNA may contribute to this IRES' mechanism of action."],"dc:identifier.doi":["10.22215/etd/2019-13701"],"dc:identifier.uri":["https://hdl.handle.net/20.500.14718/40407"],"dc:language.iso":["en"],"dc:publisher":["Carleton University"],"dc:rights":["Copyright © 2019 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. 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