Identification and Investigation of Novel DNA Damage Repair Genes Via Network Analysis

While most DNA lesions are repaired faithfully and without genotoxic effect, double-stranded breaks (DSBs) are exceptional. The deleterious potential of a misrepaired DSB represents a severe threat to cellular integrity. Repair machinery defects are frequently observed in tumorigenic and oncogenic cells. General DNA damage repair mechanisms involve homologous recombination (HR), or non-homologous end joining (NHEJ). Ongoing identification of new players in DSB repair leads us to believe there are more undiscovered genes in this pathway. The highly complex and conserved nature of DSB repair across eukaryotic and mammalian cells presents an opportunity for identification of novel genes through a computationally-directed approach. Employing a 'guilt-by-association' model, we analyzed experimental and predicted interaction networks in S. cerevisiae to identify previously uncharacterized genes involved in repair. Three novel genes were discovered to influence repair- GAL7, YHI9, and YMR130W. The results of this study implicate all three in the DNA damage response network.

Name thesis:degree_name

Master of Science (M.Sc.)

Level thesis:degree_level

Master's

Discipline thesis:degree_discipline

Biology

Grantor dc:publisher

Carleton University

Year dc:date.issued

2018

Author dc:creator

• Potter, Taylor Alexandra

Statement dc:rights

• Copyright © 2018 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner.

Language dc:language.iso

en

DOI

10.22215/etd/2018-13233

OAI identifier oai:identifier

oai:carleton.scholaris.ca:20.500.14718/38841