{"id":{"repo_id":"carleton","oai_identifier":"oai:carleton.scholaris.ca:20.500.14718/27097"},"canonical_url":"https://search.dev.ndltd.org/etd/carleton/oai:carleton.scholaris.ca:20.500.14718/27097","repository":{"repo_id":"carleton","name":"Carleton University","base_url":"https://carleton.scholaris.ca/server/oai/request"},"display":{"title":"Phosphorylation of adenosine 5'-monophosphate by a dialyzed cell-free extract from escherichia Coli","abstract":"A cell-free extract of Escherichia coli, even after exhaustive dialysis, was capable of phosphorylating 5'-AMP to ADP and ATP. When cyclic AMP phosphodiesterase was present in the extract, the conversion of cyclic AMP to ADP and ATP occurred. If 3H or 32P-labeled 5'-AMP or cyclic AMP was added to the extract, a significant portion of the radioactivity was incorporated into the 3'-terminus of tRNA species. The phosphorylating capacity was sedimentable after centrifuging at 100,000 g for 3 hours. The pellet contained phosphate polymers which are neither DNA, RNA or proteins, while the supernate contained little of this material. The addition of authentic inorganic polyphosphates to the supernate restored the original phosphorylating capacity of the extract. Thus, it is concluded that the phosphorylating property is partly due to inorganic polyphosphates. A method for assaying total inorganic polyphosphates has been developed.","abstract_html":"A cell-free extract of Escherichia coli, even after exhaustive dialysis, was capable of phosphorylating 5'-AMP to ADP and ATP. When cyclic AMP phosphodiesterase was present in the extract, the conversion of cyclic AMP to ADP and ATP occurred. If 3H or 32P-labeled 5'-AMP or cyclic AMP was added to the extract, a significant portion of the radioactivity was incorporated into the 3'-terminus of tRNA species. The phosphorylating capacity was sedimentable after centrifuging at 100,000 g for 3 hours. The pellet contained phosphate polymers which are neither DNA, RNA or proteins, while the supernate contained little of this material. The addition of authentic inorganic polyphosphates to the supernate restored the original phosphorylating capacity of the extract. Thus, it is concluded that the phosphorylating property is partly due to inorganic polyphosphates. A method for assaying total inorganic polyphosphates has been developed.","abstract_has_math":false,"creators":["Chalykoff, Penu Peneff"],"institution":"Carleton University","degree_name":"Master of Science (M.Sc.)","degree_level":"Master's","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1978,"date_issued":"1978","date_published":"1978","updated_at":"2026-07-24T01:34:41Z","subjects":[],"languages":["en"],"rights":["Copyright © 1978 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["10.22215/etd/1978-12419"],"render_values":[{"text":"10.22215/etd/1978-12419","href":"https://doi.org/10.22215/etd/1978-12419","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14718/27097","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Chalykoff, Penu Peneff"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-04-08T16:22:30Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-04-08T16:22:30Z"]},{"key":"dc:date.issued","label":"Date","values":["1978"]},{"key":"dc:publisher","label":"Institution","values":["Carleton University"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master's"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (M.Sc.)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright © 1978 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["10.22215/etd/1978-12419"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14718/27097"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A cell-free extract of Escherichia coli, even after exhaustive dialysis, was capable of phosphorylating 5'-AMP to ADP and ATP. When cyclic AMP phosphodiesterase was present in the extract, the conversion of cyclic AMP to ADP and ATP occurred. If 3H or 32P-labeled 5'-AMP or cyclic AMP was added to the extract, a significant portion of the radioactivity was incorporated into the 3'-terminus of tRNA species. The phosphorylating capacity was sedimentable after centrifuging at 100,000 g for 3 hours. The pellet contained phosphate polymers which are neither DNA, RNA or proteins, while the supernate contained little of this material. The addition of authentic inorganic polyphosphates to the supernate restored the original phosphorylating capacity of the extract. Thus, it is concluded that the phosphorylating property is partly due to inorganic polyphosphates. A method for assaying total inorganic polyphosphates has been developed."]},{"key":"dc:title","label":"Title","values":["Phosphorylation of adenosine 5'-monophosphate by a dialyzed cell-free extract from escherichia Coli"]}]}],"canonical_facts":{"dc:creator":["Chalykoff, Penu Peneff"],"dc:date.accessioned":["2025-04-08T16:22:30Z"],"dc:date.available":["2025-04-08T16:22:30Z"],"dc:date.issued":["1978"],"dc:description.abstract":["A cell-free extract of Escherichia coli, even after exhaustive dialysis, was capable of phosphorylating 5'-AMP to ADP and ATP. When cyclic AMP phosphodiesterase was present in the extract, the conversion of cyclic AMP to ADP and ATP occurred. If 3H or 32P-labeled 5'-AMP or cyclic AMP was added to the extract, a significant portion of the radioactivity was incorporated into the 3'-terminus of tRNA species. The phosphorylating capacity was sedimentable after centrifuging at 100,000 g for 3 hours. The pellet contained phosphate polymers which are neither DNA, RNA or proteins, while the supernate contained little of this material. The addition of authentic inorganic polyphosphates to the supernate restored the original phosphorylating capacity of the extract. Thus, it is concluded that the phosphorylating property is partly due to inorganic polyphosphates. A method for assaying total inorganic polyphosphates has been developed."],"dc:identifier.doi":["10.22215/etd/1978-12419"],"dc:identifier.uri":["https://hdl.handle.net/20.500.14718/27097"],"dc:language.iso":["en"],"dc:publisher":["Carleton University"],"dc:rights":["Copyright © 1978 the author(s). Theses may be used for non-commercial research, educational, or related academic purposes only. Such uses include personal study, research, scholarship, and teaching. Theses may only be shared by linking to Carleton University Institutional Repository and no part may be used without proper attribution to the author. No part may be used for commercial purposes directly or indirectly via a for-profit platform; no adaptation or derivative works are permitted without consent from the copyright owner."],"dc:title":["Phosphorylation of adenosine 5'-monophosphate by a dialyzed cell-free extract from escherichia Coli"],"dc:type":["thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Master's"],"thesis:degree_name":["Master of Science (M.Sc.)"]},"updated_at":"2026-07-24T01:34:41Z"}