{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/9740"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/9740","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut","abstract":"Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides.","abstract_html":"Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides.","abstract_has_math":false,"creators":["Russouw, Tracy Karen Joe"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Thomson, Jennifer Ann","Reid, Sharon J"],"committee_chairs":[],"committee_members":[],"year":2000,"date_issued":"2000","date_published":"2000","updated_at":"2026-07-22T22:23:18Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/9740","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Thomson, Jennifer Ann","Reid, Sharon J"]},{"key":"dc:creator","label":"Author","values":["Russouw, Tracy Karen Joe"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-11-20T03:57:34Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-11-20T03:57:34Z"]},{"key":"dc:date.issued","label":"Date","values":["2000"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MPhil"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/9740"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes bibliographical references."]},{"key":"dc:description.abstract","label":"Abstract","values":["Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides."]},{"key":"dc:title","label":"Title","values":["Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut"]}]}],"canonical_facts":{"dc:contributor.advisor":["Thomson, Jennifer Ann","Reid, Sharon J"],"dc:creator":["Russouw, Tracy Karen Joe"],"dc:date.accessioned":["2014-11-20T03:57:34Z"],"dc:date.available":["2014-11-20T03:57:34Z"],"dc:date.issued":["2000"],"dc:description":["Includes bibliographical references."],"dc:description.abstract":["Eleven bacterial strains that have been isolated from the ostrich hindgut, have successfully been identified with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript (SK). The results obtained from the 16S rDNA sequence homologies strongly suggests that the newly identified bacteria should be assigned to three major bacterial genera, namely, Ruminococcus, Buryrivibrio and Bacteroides. Four highly cellulolytic strains were identified as ruminococci while two weakly cellulolytic strains, showed highest homology to members of the genus Butyrivibrio. Another weakly cellulolytic strain showed closest identity to the sulfur reducing bacterial genus Desulfovibrio. Four non-cellulolytic strains showed highest homology to the genus Bacteroides."],"dc:identifier.uri":["http://hdl.handle.net/11427/9740"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MPhil"]},"updated_at":"2026-07-22T22:23:18Z"}