Isolation and molecular characterisation of two Pseudomonas sp. ACC deaminase genes

The phytohormone ethylene is essential to many plant developmental processes, of which the control of climacteric fruit ripening is among the best characterised. However this hormone eventually causes fruit rotting which results in a non-marketable product. One approach to reduce ethylene synthesis in plants is metabolism of its immediate precursor, 1-aminocyclopropane-1carboxylic acid (ACC). This can be achieved through degradation of ACC by the enzyme ACC deaminase to form α-ketobutyric acid and ammonia. ACC degrading soil microorganisms were identified by their ability to grow on ACC as a sole nitrogen source. Enzyme assays indicated that Pseudomonas had high ACC deaminase gene-specific primers and probes respectively revealed that only one bacterium, Pseudomonas fluerescens strain 17, had a gene with homology to previously sequenced ACC deaminase genes.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

1995

Author dc:creator

• Campbell, Bridget Genevieve

Advisors dc:contributor.advisor

• Thomson, Jennifer Ann
• Brand Reon

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/9650

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/9650