Molecular characterization of XvPer1, a novel antioxidant enzyme from the resurrection plant Xerophyta viscosa, and AC3, a LEA-like protein from Arabidopsis thaliana

By differential screening of a cDNA library of the resurrection plant Xerophyta viscosa, a cDNA termed XvPer I was isolated for its over-expression under dehydration stress. Analysis of the cDNA sequence indicated a hydrophilic protein of a predicted Mw of 24.2 kDa with high identity to plant l-Cys peroxiredoxins, a novel antioxidant enzyme. Southern blot analysis revealed that XvPer I was most probably a single copy gene. The polypeptide sequence had significant identities (-70%) with other recently identified plant I-Cys peroxiredoxins with an absolutely conserved active site (PVCTTE). The protein sequence also had a putative bipartite nuclear localization signal.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2005

Author dc:creator

• Mowla, Shaheen B

Advisors dc:contributor.advisor

• Mundree, Sagadevan G
• Thomson, Jennifer Ann
• Farrant, Jill M
• Foyer, C H
• Theodoulou, F

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/6728

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/6728