{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/5970"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/5970","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Development of candidate Human papillomavirus vaccines","abstract":"The objective of this thesis was to investigate novel and plant-based vaccines against the Human papillomavirus type 16 (HPV-16), which is primarily responsible for cervical cancer. As a first study, the L1 gene of a South Africna variant of HPV-16 (L1 504) and a mutant (504[ΔA266T]), where the alanine at 266 was mutated to a threonine, were expressed in insect cells by recombinant baculovirus, and the resulting virus-like particles (VLPs) were tested with a panel of well-characterised monoclonal antibodies (Mabs).","abstract_html":"The objective of this thesis was to investigate novel and plant-based vaccines against the Human papillomavirus type 16 (HPV-16), which is primarily responsible for cervical cancer. As a first study, the L1 gene of a South Africna variant of HPV-16 (L1 504) and a mutant (504[ΔA266T]), where the alanine at 266 was mutated to a threonine, were expressed in insect cells by recombinant baculovirus, and the resulting virus-like particles (VLPs) were tested with a panel of well-characterised monoclonal antibodies (Mabs).","abstract_has_math":false,"creators":["Varsani, Arvind"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Rybicki, Ed","Williamson, Anne-Lise"],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003","date_published":"2003","updated_at":"2026-07-22T22:22:46Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/5970","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Rybicki, Ed","Williamson, Anne-Lise"]},{"key":"dc:creator","label":"Author","values":["Varsani, Arvind"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-08-02T14:56:43Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-08-02T14:56:43Z"]},{"key":"dc:date.issued","label":"Date","values":["2003"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/5970"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Bibliography: leaves 180-218."]},{"key":"dc:description.abstract","label":"Abstract","values":["The objective of this thesis was to investigate novel and plant-based vaccines against the Human papillomavirus type 16 (HPV-16), which is primarily responsible for cervical cancer. As a first study, the L1 gene of a South Africna variant of HPV-16 (L1 504) and a mutant (504[ΔA266T]), where the alanine at 266 was mutated to a threonine, were expressed in insect cells by recombinant baculovirus, and the resulting virus-like particles (VLPs) were tested with a panel of well-characterised monoclonal antibodies (Mabs)."]},{"key":"dc:title","label":"Title","values":["Development of candidate Human papillomavirus vaccines"]}]}],"canonical_facts":{"dc:contributor.advisor":["Rybicki, Ed","Williamson, Anne-Lise"],"dc:creator":["Varsani, Arvind"],"dc:date.accessioned":["2014-08-02T14:56:43Z"],"dc:date.available":["2014-08-02T14:56:43Z"],"dc:date.issued":["2003"],"dc:description":["Bibliography: leaves 180-218."],"dc:description.abstract":["The objective of this thesis was to investigate novel and plant-based vaccines against the Human papillomavirus type 16 (HPV-16), which is primarily responsible for cervical cancer. 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