The Laccase from Micromonospora sp.044 30-1 as a biocatalyst for synthesis of antioxidant compounds
type: CreativeWork
description: Laccases (EC 1.10.3.2) are blue multicopper oxidases that catalyse a single electron oxidation of various phenolic substrates with an associated four-electron reduction of dioxygen to water. The varied uses of laccase as a biocatalyst can be attributed to its ability to produce a free radical from a suitable substrate. Of importance to the pharmaceutical, chemical, and industrial sectors, are the laccase-catalysed reactions providing means for the synthesis of dimeric phenolics showing biological activity, including antioxidant activity. The objective of this research was to investigate the production of value-added compounds, with biological activity, via laccase-catalysed oxidation reactions. Our laboratory has access to several unique and previously unexploited culture collections obtained from extreme environments spanning the globe. Potentially novel strains were screened for the ability to produce laccases: 14 environmental isolates, of which 2 strains were fungi, 7 were streptomycetes, and 5 were non-streptomycetes, representing the rare actinomycete genera Gordonia, Rhodococcus, Mycobacterium, Amycolatopsis, and Micromonospora were screened. This is the first report of laccase production in these species. A screening protocol, using criteria specifically suited to bioprocess development, was developed to investigate variables affecting the production of laccase by the native strains. Variables investigated included different types of media (nutritional variables), pH, temperature, incubation times, aeration and agitation, salt concentrations, and the effect of inducers on laccase production by the native strains. Of the isolates investigated, actinomycete strain Micromonospora sp. 044 30-1 showed the greatest potential for the production of laccase. This strain may be novel and the role of laccase in this strain may be related to sporulation. Various growth requirements were investigated in order to optimise for maximal laccase production by strain 044 30-1.…
abstract: Laccases (EC 1.10.3.2) are blue multicopper oxidases that catalyse a single electron oxidation of various phenolic substrates with an associated four-electron reduction of dioxygen to water. The varied uses of laccase as a biocatalyst can be attributed to its ability to produce a free radical from a suitable substrate. Of importance to the pharmaceutical, chemical, and industrial sectors, are the laccase-catalysed reactions providing means for the synthesis of dimeric phenolics showing biological activity, including antioxidant activity. The objective of this research was to investigate the production of value-added compounds, with biological activity, via laccase-catalysed oxidation reactions. Our laboratory has access to several unique and previously unexploited culture collections obtained from extreme environments spanning the globe. Potentially novel strains were screened for the ability to produce laccases: 14 environmental isolates, of which 2 strains were fungi, 7 were streptomycetes, and 5 were non-streptomycetes, representing the rare actinomycete genera Gordonia, Rhodococcus, Mycobacterium, Amycolatopsis, and Micromonospora were screened. This is the first report of laccase production in these species. A screening protocol, using criteria specifically suited to bioprocess development, was developed to investigate variables affecting the production of laccase by the native strains. Variables investigated included different types of media (nutritional variables), pH, temperature, incubation times, aeration and agitation, salt concentrations, and the effect of inducers on laccase production by the native strains. Of the isolates investigated, actinomycete strain Micromonospora sp. 044 30-1 showed the greatest potential for the production of laccase. This strain may be novel and the role of laccase in this strain may be related to sporulation. Various growth requirements were investigated in order to optimise for maximal laccase production by strain 044 30-1.…
author: Goodwin, Candice Michelle
language: eng
date published: 2010
date modified: 2026-07-22T22:23:41Z
provider: University of Cape Town
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Centre for Bioprocess Engineering Research

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property ID: Handle
value: http://hdl.handle.net/11427/5329