Expression of HPV-16 L2 in plants

This study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2008

Author dc:creator

• Pereira, Roman

Advisors dc:contributor.advisor

• Rybicki, Ed
• Becker, Inga
• Maclean, James

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/4317

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/4317