{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/4288"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/4288","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"DNA based networks, nanostructures, and meta-stable states","abstract":"Nanotechnology is defined as the technology that allows to fabricate nanoscale materials and manipulate them at the molecular or atomic level. Non-biotechnology is one of the sub-disciplines of nanotechnology. Materials used for nano-biotechnology are macromolecular materials found in nature with specific mechanical and biological functions. One of the most important materials is deoxyribonucleic acid (DNA). DNA has several unique properties that make it a good candidate for nanotechnology research. The primary aim of this research is to use DNA three-way junctions as building blocks and to assemble them into a huge DNA networks through self assembly followed by DNA ligation. The second step aims of assembling six DNA three-way junctions with different sticky end sequences to from an opto-mechanical hexagonal DNA nano-switch. The switching mechanism in the righ is based on the conformational change of B- to Z- DNA. This change is influenced by the environmental cation concentrations. The third approach presented here aims to create meta-stable DNA nanostructures based on a DNA hairpin loop that can mimic the enzyme-substrate binding mechanism. This mechanism is demonstrated using a \"strand displacement strategy\" (SDS). Lastly, we employed a novel way of organizing hairpin loop DNA structures based on the orientation of consecutive hairpin loop DNA structures \"on top\" of one another.","abstract_html":"Nanotechnology is defined as the technology that allows to fabricate nanoscale materials and manipulate them at the molecular or atomic level. Non-biotechnology is one of the sub-disciplines of nanotechnology. Materials used for nano-biotechnology are macromolecular materials found in nature with specific mechanical and biological functions. One of the most important materials is deoxyribonucleic acid (DNA). DNA has several unique properties that make it a good candidate for nanotechnology research. The primary aim of this research is to use DNA three-way junctions as building blocks and to assemble them into a huge DNA networks through self assembly followed by DNA ligation. The second step aims of assembling six DNA three-way junctions with different sticky end sequences to from an opto-mechanical hexagonal DNA nano-switch. The switching mechanism in the righ is based on the conformational change of B- to Z- DNA. This change is influenced by the environmental cation concentrations. The third approach presented here aims to create meta-stable DNA nanostructures based on a DNA hairpin loop that can mimic the enzyme-substrate binding mechanism. This mechanism is demonstrated using a "strand displacement strategy" (SDS). Lastly, we employed a novel way of organizing hairpin loop DNA structures based on the orientation of consecutive hairpin loop DNA structures "on top" of one another.","abstract_has_math":false,"creators":["Lin, Chih-Ta"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Klump, HH"],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006","date_published":"2006","updated_at":"2026-07-22T22:22:43Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/4288","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Klump, HH"]},{"key":"dc:creator","label":"Author","values":["Lin, Chih-Ta"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-30T17:38:53Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-30T17:38:53Z"]},{"key":"dc:date.issued","label":"Date","values":["2006"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/4288"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes bibliographical references (leaves 162-170)."]},{"key":"dc:description.abstract","label":"Abstract","values":["Nanotechnology is defined as the technology that allows to fabricate nanoscale materials and manipulate them at the molecular or atomic level. Non-biotechnology is one of the sub-disciplines of nanotechnology. Materials used for nano-biotechnology are macromolecular materials found in nature with specific mechanical and biological functions. One of the most important materials is deoxyribonucleic acid (DNA). DNA has several unique properties that make it a good candidate for nanotechnology research. The primary aim of this research is to use DNA three-way junctions as building blocks and to assemble them into a huge DNA networks through self assembly followed by DNA ligation. The second step aims of assembling six DNA three-way junctions with different sticky end sequences to from an opto-mechanical hexagonal DNA nano-switch. The switching mechanism in the righ is based on the conformational change of B- to Z- DNA. This change is influenced by the environmental cation concentrations. The third approach presented here aims to create meta-stable DNA nanostructures based on a DNA hairpin loop that can mimic the enzyme-substrate binding mechanism. This mechanism is demonstrated using a \"strand displacement strategy\" (SDS). Lastly, we employed a novel way of organizing hairpin loop DNA structures based on the orientation of consecutive hairpin loop DNA structures \"on top\" of one another."]},{"key":"dc:title","label":"Title","values":["DNA based networks, nanostructures, and meta-stable states"]}]}],"canonical_facts":{"dc:contributor.advisor":["Klump, HH"],"dc:creator":["Lin, Chih-Ta"],"dc:date.accessioned":["2014-07-30T17:38:53Z"],"dc:date.available":["2014-07-30T17:38:53Z"],"dc:date.issued":["2006"],"dc:description":["Includes bibliographical references (leaves 162-170)."],"dc:description.abstract":["Nanotechnology is defined as the technology that allows to fabricate nanoscale materials and manipulate them at the molecular or atomic level. Non-biotechnology is one of the sub-disciplines of nanotechnology. Materials used for nano-biotechnology are macromolecular materials found in nature with specific mechanical and biological functions. One of the most important materials is deoxyribonucleic acid (DNA). DNA has several unique properties that make it a good candidate for nanotechnology research. The primary aim of this research is to use DNA three-way junctions as building blocks and to assemble them into a huge DNA networks through self assembly followed by DNA ligation. The second step aims of assembling six DNA three-way junctions with different sticky end sequences to from an opto-mechanical hexagonal DNA nano-switch. The switching mechanism in the righ is based on the conformational change of B- to Z- DNA. This change is influenced by the environmental cation concentrations. The third approach presented here aims to create meta-stable DNA nanostructures based on a DNA hairpin loop that can mimic the enzyme-substrate binding mechanism. This mechanism is demonstrated using a \"strand displacement strategy\" (SDS). Lastly, we employed a novel way of organizing hairpin loop DNA structures based on the orientation of consecutive hairpin loop DNA structures \"on top\" of one another."],"dc:identifier.uri":["http://hdl.handle.net/11427/4288"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["DNA based networks, nanostructures, and meta-stable states"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:43Z"}