Polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinaemia (Type III hyperlipidaemia)

Accurate phenotypic diagnosis of this disorder has generally relied on the quantitative analysis of centrifugally isolated VLDL. Analysis of VLDL chemical composition is not widely available due to the expense of acquiring ultracentrifuges and the highly-skilled staff necessary to perform the analyses. Genotypic diagnosis is somewhat more widely available, especially testing for the E2/E2 genotype which is the commonest underlying genotype in dysbetalipoproteinaemia. Testing for the rarer autosomal mutations of ApoE is restricted to a small number of centres only. In this study I will review dysbetalipoproteinaemia briefly and then describe and evaluate GGE as a new diagnostic technique for this disorder.

Grantor dc:publisher.institution

Department of Medicine

Year dc:date.issued

2001

Author dc:creator

• Blom, Dirk Jacobus

Advisor dc:contributor.advisor

• Marais, David

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/3365

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/3365