{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/3301"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/3301","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Characterising the role of actin and PI (3) kinases in endocytosis in the malaria parasite Plasmodium falciparum","abstract":"By contrast to mammalian cells, very little is known about endocytosis in the malaria parasite. However, endocytosis via the cytostome is required by the parasite to ingest haemoglobin from its host cytosol which it transports within double membrane vesicles to the digestive vacuole, where digestion occurs and metabolites are used mostly for nutritional purposes. To gain a deeper understanding of the molecular basis and mechanisms of this vital process, a panel of inhibitors was used to inhibit the actin cytoskeleton and PI (3) kinases in the parasite. In this study Cytochalasin D and Latrunculin A, which depolymerise and prevent actin fimalment formation, Jasplakinolide, which stabilises actin filaments, and Wormannin and LY294002, which inhibit PI 93) kinase, were used to study actin disrupting and PI (3) kinase inhibiting drug effects on haemoglobin endocytosis and transport vesicle trafficking within the malaria parasite Plasmodium falciparum.","abstract_html":"By contrast to mammalian cells, very little is known about endocytosis in the malaria parasite. However, endocytosis via the cytostome is required by the parasite to ingest haemoglobin from its host cytosol which it transports within double membrane vesicles to the digestive vacuole, where digestion occurs and metabolites are used mostly for nutritional purposes. To gain a deeper understanding of the molecular basis and mechanisms of this vital process, a panel of inhibitors was used to inhibit the actin cytoskeleton and PI (3) kinases in the parasite. In this study Cytochalasin D and Latrunculin A, which depolymerise and prevent actin fimalment formation, Jasplakinolide, which stabilises actin filaments, and Wormannin and LY294002, which inhibit PI 93) kinase, were used to study actin disrupting and PI (3) kinase inhibiting drug effects on haemoglobin endocytosis and transport vesicle trafficking within the malaria parasite Plasmodium falciparum.","abstract_has_math":false,"creators":["Smythe, Wynand Anton"],"institution":"Division of Clinical Pharmacology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Hoppe, Heinrich C"],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-22T22:22:39Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/3301","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Hoppe, Heinrich C"]},{"key":"dc:creator","label":"Author","values":["Smythe, Wynand Anton"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-28T18:20:52Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-28T18:20:52Z"]},{"key":"dc:date.issued","label":"Date","values":["2007"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Clinical Pharmacology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/3301"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes bibliographical references (leaves 94-103)."]},{"key":"dc:description.abstract","label":"Abstract","values":["By contrast to mammalian cells, very little is known about endocytosis in the malaria parasite. However, endocytosis via the cytostome is required by the parasite to ingest haemoglobin from its host cytosol which it transports within double membrane vesicles to the digestive vacuole, where digestion occurs and metabolites are used mostly for nutritional purposes. To gain a deeper understanding of the molecular basis and mechanisms of this vital process, a panel of inhibitors was used to inhibit the actin cytoskeleton and PI (3) kinases in the parasite. In this study Cytochalasin D and Latrunculin A, which depolymerise and prevent actin fimalment formation, Jasplakinolide, which stabilises actin filaments, and Wormannin and LY294002, which inhibit PI 93) kinase, were used to study actin disrupting and PI (3) kinase inhibiting drug effects on haemoglobin endocytosis and transport vesicle trafficking within the malaria parasite Plasmodium falciparum."]},{"key":"dc:title","label":"Title","values":["Characterising the role of actin and PI (3) kinases in endocytosis in the malaria parasite Plasmodium falciparum"]}]}],"canonical_facts":{"dc:contributor.advisor":["Hoppe, Heinrich C"],"dc:creator":["Smythe, Wynand Anton"],"dc:date.accessioned":["2014-07-28T18:20:52Z"],"dc:date.available":["2014-07-28T18:20:52Z"],"dc:date.issued":["2007"],"dc:description":["Includes bibliographical references (leaves 94-103)."],"dc:description.abstract":["By contrast to mammalian cells, very little is known about endocytosis in the malaria parasite. However, endocytosis via the cytostome is required by the parasite to ingest haemoglobin from its host cytosol which it transports within double membrane vesicles to the digestive vacuole, where digestion occurs and metabolites are used mostly for nutritional purposes. To gain a deeper understanding of the molecular basis and mechanisms of this vital process, a panel of inhibitors was used to inhibit the actin cytoskeleton and PI (3) kinases in the parasite. In this study Cytochalasin D and Latrunculin A, which depolymerise and prevent actin fimalment formation, Jasplakinolide, which stabilises actin filaments, and Wormannin and LY294002, which inhibit PI 93) kinase, were used to study actin disrupting and PI (3) kinase inhibiting drug effects on haemoglobin endocytosis and transport vesicle trafficking within the malaria parasite Plasmodium falciparum."],"dc:identifier.uri":["http://hdl.handle.net/11427/3301"],"dc:language.iso":["eng"],"dc:publisher.department":["Division of Clinical Pharmacology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Characterising the role of actin and PI (3) kinases in endocytosis in the malaria parasite Plasmodium falciparum"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc"]},"updated_at":"2026-07-22T22:22:39Z"}