Platelet function Analyzer; closure times in children with congenital cyanotic heart disease A prospective observational pilot study

Objectives: To establish the median and interquartile range or the mean and standard deviation for closure times , with the CADP and CEPI cartridges for children with CCHD and to compare this to normal children. Design: Prospective observational pilot study Setting: Red Cross War Memorial Children's Hospital (RCWMCH) in association with the University of Cape Town Participants: Children between birth and 16 years old diagnosed with CCHD presenting for corrective or palliative cardiothoracic surgical procedures Interventions: 0.8ml of whole blood obtained from the participants was pipetted into both the CEPI and CADP cartridges and analyzed by the PFA machine. Closure times for both cartridges were obtained and recorded on the data collection form. Results: 40 successful CADP samples and 39 successful CEPI cartridges were analysed. Of the total 40 valid CADP samples there was left skewed distribution , the median was 114.50 seconds with an interquartile range from 87.25 seconds to 153.75 seconds. Of the total 39 valid CEPI samples the data was normally distributed to give a mean of 175.38 and a standard deviation of 74.998. Both of which are not significantly different from the typical normal ranges obtained with 3.2% trisodium citrate ; 55–112 s for CADP and 79–164 s for CEPI (Harrison 2005). However, when compared to the normal ranges quoted by Carcao et al for neonates and children, there was a significant prolongation for both the CEPI and CADP samples in the neonates and children with CCHD Conclusion: This is a pilot study and limited by small sample sizes obtained due to time limitation. Further research would be needed to further assess whether the PFA could be used to guide platelet replacement in this population.

Grantor

Department of Anaesthesia and Perioperative Medicine

Year dc:date.issued

2020

Author dc:creator

• Kempe, Laura Jessica

Advisor dc:contributor.advisor

• Wilson, Graeme

• Perioperative Medicine and Anaesthesia

Handle dc:identifier.uri

http://hdl.handle.net/11427/32749

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/32749