The use of histological examination methods to distinguish between the burnt remains of human and non-human bones

Distinguishing between burnt human and non-human bone fragments using macroscopic methods has proved challenging and it was seen in the literature review that the previous research did not all come to the same conclusions. The aim of the research was to determine if, using histological methods, it was possible to distinguish between burned human and nonhuman bone fragments. A literature review was compiled to provide an overview of the anatomy of bones, morphological characteristics of bone, general bone histology, a comparison between human and non-human bones and the effect of temperature on bones. Bones of five different species (human, baboon, wildebeest, pig and cow) were burned in a muffle furnace for twenty minutes at either 600ᵒC or 800ᵒC. Following the burning procedure, thin ground bone sections of the burned and unburned bone specimens were prepared for microscopic analysis and the minimum canal diameter, maximum canal diameter, minimum Haversian system diameter, maximum Haversian system diameter, area of canal, and area of the Haversian system were measured. A comparative analysis was then done across species and temperature. A total of 523 osteons in unburned bone and 147 in the burned bone samples were analysed. ANOVA testing found overall significance (p < 0.0001) for all parameters measured, which suggests that temperature does affect the size of microstructures. Most parameter sizes increased with an increase in temperature. A greater increase was seen at 600 degrees than 800 degrees. Qualitatively, carbonation within the burned bone, made the measurement of parameters difficult in some samples. Human bone can easily be differentiated from pig, cow or wildebeest bone due to no or very few osteons present. Pig bone consisted almost entirely of plexiform bone, while the cow and wildebeest presented with only a few osteons in some parts of the bone. Human and baboon bone appeared similar on a microscopic level. The study revealed that temperature did not, in general, hamper the ability to differentiate between burned human and non-human bone, but it did impact on the number of measurable data points for each parameter.

Grantor dc:publisher

University of Cape Town

Year dc:date.issued

2020

Author dc:creator

• Labuschagne, Lizl

Advisors dc:contributor.advisor

• Mole, Calvin
• Heyns, Marise

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/32470

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/32470