{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/3067"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/3067","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"An in vitro investigation into the pigmentary phenotype of melanocytes and keratinocyte co-cultures to improve wound healing","abstract":"On healing, partial-thickness burn wounds usually result in depigmentation of the skin. This is due to the loss of melanocytes. The lack of pigmentation in the healed wound is particularly prominent in dark-skinned individuals and could result in serious psychosocial consequences such as low self-esteem, stigmatisation and discrimination among sufferers. Methods aimed at investigating rapid and efficient repigmentation in wounded skin are therefore pertinent. The aims of this study were two-fold: i) To promote melanin synthesis in human skin cells using different ratios of human melanocytes (Mc) to keratinocytes (Kc) in an in vitro co-culture system, in order to ensure pigmentation of the skin and, ii) To understand cellular mechanisms that contribute basic scientific knowledge towards clinically improved wound healing.","abstract_html":"On healing, partial-thickness burn wounds usually result in depigmentation of the skin. This is due to the loss of melanocytes. The lack of pigmentation in the healed wound is particularly prominent in dark-skinned individuals and could result in serious psychosocial consequences such as low self-esteem, stigmatisation and discrimination among sufferers. Methods aimed at investigating rapid and efficient repigmentation in wounded skin are therefore pertinent. The aims of this study were two-fold: i) To promote melanin synthesis in human skin cells using different ratios of human melanocytes (Mc) to keratinocytes (Kc) in an in vitro co-culture system, in order to ensure pigmentation of the skin and, ii) To understand cellular mechanisms that contribute basic scientific knowledge towards clinically improved wound healing.","abstract_has_math":false,"creators":["Chang, Ju-Wei"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Davids, Lester M"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013","date_published":"2013","updated_at":"2026-07-22T22:22:47Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/3067","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Davids, Lester M"]},{"key":"dc:creator","label":"Author","values":["Chang, Ju-Wei"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-28T14:50:22Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-28T14:50:22Z"]},{"key":"dc:date.issued","label":"Date","values":["2013"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/3067"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes abstract.","Includes bibliographical references."]},{"key":"dc:description.abstract","label":"Abstract","values":["On healing, partial-thickness burn wounds usually result in depigmentation of the skin. This is due to the loss of melanocytes. The lack of pigmentation in the healed wound is particularly prominent in dark-skinned individuals and could result in serious psychosocial consequences such as low self-esteem, stigmatisation and discrimination among sufferers. Methods aimed at investigating rapid and efficient repigmentation in wounded skin are therefore pertinent. The aims of this study were two-fold: i) To promote melanin synthesis in human skin cells using different ratios of human melanocytes (Mc) to keratinocytes (Kc) in an in vitro co-culture system, in order to ensure pigmentation of the skin and, ii) To understand cellular mechanisms that contribute basic scientific knowledge towards clinically improved wound healing."]},{"key":"dc:title","label":"Title","values":["An in vitro investigation into the pigmentary phenotype of melanocytes and keratinocyte co-cultures to improve wound healing"]}]}],"canonical_facts":{"dc:contributor.advisor":["Davids, Lester M"],"dc:creator":["Chang, Ju-Wei"],"dc:date.accessioned":["2014-07-28T14:50:22Z"],"dc:date.available":["2014-07-28T14:50:22Z"],"dc:date.issued":["2013"],"dc:description":["Includes abstract.","Includes bibliographical references."],"dc:description.abstract":["On healing, partial-thickness burn wounds usually result in depigmentation of the skin. This is due to the loss of melanocytes. The lack of pigmentation in the healed wound is particularly prominent in dark-skinned individuals and could result in serious psychosocial consequences such as low self-esteem, stigmatisation and discrimination among sufferers. Methods aimed at investigating rapid and efficient repigmentation in wounded skin are therefore pertinent. The aims of this study were two-fold: i) To promote melanin synthesis in human skin cells using different ratios of human melanocytes (Mc) to keratinocytes (Kc) in an in vitro co-culture system, in order to ensure pigmentation of the skin and, ii) To understand cellular mechanisms that contribute basic scientific knowledge towards clinically improved wound healing."],"dc:identifier.uri":["http://hdl.handle.net/11427/3067"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["An in vitro investigation into the pigmentary phenotype of melanocytes and keratinocyte co-cultures to improve wound healing"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc"]},"updated_at":"2026-07-22T22:22:47Z"}