{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/27657"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/27657","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats","abstract":"The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20).","abstract_html":"The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20).","abstract_has_math":false,"creators":["Shapiro, Brahm"],"institution":"Division of Endocrinology and Diabetology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Pimstone, Bernard L"],"committee_chairs":[],"committee_members":[],"year":1978,"date_issued":"1978","date_published":"1978","updated_at":"2026-07-22T22:22:50Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/27657","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Pimstone, Bernard L"]},{"key":"dc:creator","label":"Author","values":["Shapiro, Brahm"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-03-15T07:38:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-03-15T07:38:00Z"]},{"key":"dc:date.issued","label":"Date","values":["1978"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Endocrinology and Diabetology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/27657"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. 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The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20)."],"dc:identifier.uri":["http://hdl.handle.net/11427/27657"],"dc:language.iso":["eng"],"dc:publisher.department":["Division of Endocrinology and Diabetology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:50Z"}