{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/2727"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/2727","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector","abstract":"This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. 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Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly.","abstract_has_math":false,"creators":["Griffin, Sarah"],"institution":"Division of Virology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Chapman, Rosamund"],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-24T01:33:49Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/2727","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Chapman, Rosamund"]},{"key":"dc:creator","label":"Author","values":["Griffin, Sarah"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-28T08:19:16Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-28T08:19:16Z"]},{"key":"dc:date.issued","label":"Date","values":["2007"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Virology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/2727"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Includes bibliographical references (leaves 89-95)."]},{"key":"dc:description.abstract","label":"Abstract","values":["This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly."]},{"key":"dc:title","label":"Title","values":["Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector"]}]}],"canonical_facts":{"dc:contributor.advisor":["Chapman, Rosamund"],"dc:creator":["Griffin, Sarah"],"dc:date.accessioned":["2014-07-28T08:19:16Z"],"dc:date.available":["2014-07-28T08:19:16Z"],"dc:date.issued":["2007"],"dc:description":["Includes bibliographical references (leaves 89-95)."],"dc:description.abstract":["This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly."],"dc:identifier.uri":["http://hdl.handle.net/11427/2727"],"dc:language.iso":["eng"],"dc:publisher.department":["Division of Virology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc"]},"updated_at":"2026-07-24T01:33:49Z"}