Birds of a white feather : a congenital hypopigmentary disorder in the chick
type: CreativeWork
description: Dominant white, a mutation of the I gene, leads to amelanosis and is common to many commercial breeds of fowl, including Pile Games, White Plymouth Rocks and White Leghorns. Despite much investigation on the cellular mechanisms of Dominant white, its mode of action is still poorly understood. The aim of this study is to elucidate the molecular basis of amelanosis in WPR X PG chickens by addressing the following two questions: are melanocytes present in the skin regions and feather follicles in normal numbers of 8- to 13-day white chick embryos? If melanocytes are present in normal numbers, are they unable to synthesise pigment because of a defect in melanocyte differentiation? Two approaches were used to answer the first question. MelEM, a monoclonal antibody, shown to react specifically to quail melanocytes, was found to be unsuitable for localisation of chicken melanocytes. Secondly, in situ hybridisation with tyrosinase and tyrosinase related protein-2 (TYRP2) probes was carried out to quantitate the number of melanocytes at different stages of development. The results indicate that tyrosinase - and TYRP2 -expressing melanocytes are present in 10-day white chick skin and feather buds in normal, if not greater numbers than in the control (Black Australorp) breed. This suggests that amelanosis is not due the failure of migratory melanoblasts to reach the developing feathers, nor is it due to the selective elimination of melanocytes during migration. The results further showed that with increasing developmental age (12- and 13-days), there is a decline in the number of tyrosinase - and TYRP2-expressing melanocytes in the white chick breed in comparison to the black breed. This suggests that white skin melanocytes either downregulate tyrosinase and TYRP2 gene expression yet remain viable, or they undergo cell death. At 17-days, the results showed an absence of gene expression in both the black and white follicles due to the normal process of feather development. Thus…
abstract: Dominant white, a mutation of the I gene, leads to amelanosis and is common to many commercial breeds of fowl, including Pile Games, White Plymouth Rocks and White Leghorns. Despite much investigation on the cellular mechanisms of Dominant white, its mode of action is still poorly understood. The aim of this study is to elucidate the molecular basis of amelanosis in WPR X PG chickens by addressing the following two questions: are melanocytes present in the skin regions and feather follicles in normal numbers of 8- to 13-day white chick embryos? If melanocytes are present in normal numbers, are they unable to synthesise pigment because of a defect in melanocyte differentiation? Two approaches were used to answer the first question. MelEM, a monoclonal antibody, shown to react specifically to quail melanocytes, was found to be unsuitable for localisation of chicken melanocytes. Secondly, in situ hybridisation with tyrosinase and tyrosinase related protein-2 (TYRP2) probes was carried out to quantitate the number of melanocytes at different stages of development. The results indicate that tyrosinase - and TYRP2 -expressing melanocytes are present in 10-day white chick skin and feather buds in normal, if not greater numbers than in the control (Black Australorp) breed. This suggests that amelanosis is not due the failure of migratory melanoblasts to reach the developing feathers, nor is it due to the selective elimination of melanocytes during migration. The results further showed that with increasing developmental age (12- and 13-days), there is a decline in the number of tyrosinase - and TYRP2-expressing melanocytes in the white chick breed in comparison to the black breed. This suggests that white skin melanocytes either downregulate tyrosinase and TYRP2 gene expression yet remain viable, or they undergo cell death. At 17-days, the results showed an absence of gene expression in both the black and white follicles due to the normal process of feather development. Thus…
author: Koubovec, Dominique Johanna
language: eng
date published: 1999
date modified: 2026-07-22T22:22:58Z
provider: University of Cape Town
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Division of Cell Biology

PropertyValue
property ID: Handle
value: http://hdl.handle.net/11427/26786