{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/26701"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/26701","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos)","abstract":"I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings.","abstract_html":"I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings.","abstract_has_math":false,"creators":["Potts, Alistair"],"institution":"Department of Biological Sciences","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Verboom, George Anthony","Hoffmann, Timm"],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006","date_published":"2006","updated_at":"2026-07-22T22:23:29Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/26701","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Verboom, George Anthony","Hoffmann, Timm"]},{"key":"dc:creator","label":"Author","values":["Potts, Alistair"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-12-15T07:06:49Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-12-15T07:06:49Z"]},{"key":"dc:date.issued","label":"Date","values":["2006"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Biological Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Bachelor Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Honours"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["BSc (Hons)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/26701"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings."]},{"key":"dc:title","label":"Title","values":["Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos)"]}]}],"canonical_facts":{"dc:contributor.advisor":["Verboom, George Anthony","Hoffmann, Timm"],"dc:creator":["Potts, Alistair"],"dc:date.accessioned":["2017-12-15T07:06:49Z"],"dc:date.available":["2017-12-15T07:06:49Z"],"dc:date.issued":["2006"],"dc:description.abstract":["I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings."],"dc:identifier.uri":["http://hdl.handle.net/11427/26701"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Biological Sciences"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos)"],"dc:type":["Bachelor Thesis"],"dc:type.qualificationlevel":["Honours"],"dc:type.qualificationname":["BSc (Hons)"]},"updated_at":"2026-07-22T22:23:29Z"}