Investigation of the effect of a probiotic-supplemented diet on the haemocyte proteome of the abalone Haliotis midae
type: CreativeWork
description: Haliotis midae is an economically important South African abalone species which has been cultured since the 1990s. H. midae farming constitutes approximately 93% of the country's total marine aquaculture industry. However, the slow growth rate of this animal and the potential for disease outbreaks remain a concern for farmers. Therefore, government and private institutions have joined efforts to investigate ways of enhancing abalone production, to increase abalone growth rates and improve disease resistance. Several studies have shown that probiotic microorganisms can significantly increase the growth rate and disease resistance of H. midae. To date, no comprehensive studies have been conducted to characterise these physiological improvements at the molecular level. Thus, the aim of this study was to evaluate the effect of a probiotic-supplemented diet on the haemocyte proteome in H. midae. Two probiotic-strains, Vibrio midae SY9 and Debaryomuces hansenii AY1, were introduced into H. midae via a kelp-based feed. Changes in the haemocyte proteome were analysed using isobaric tag for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS. A total of 128 haemocyte proteins were identified. Proteins that were found to vary significantly in their expression levels in haemocytes sampled from abalone fed the probiotic-supplemented diet were identified as COP9 signalosome subunit 4, phosphorylase, T-complex protein 1 subunit gamma, V-type proton ATPase subunit B, Rab 1 and Ra-related protein Rab 1A. Differential expression of COP9 signalosome subunit 4 (up-regulated) and Ras-related protein Rab 1A (down-regulated) was confirmed by western blot analysis. Bioinformatics analysis revealed proteins with immune class GO terms that functioned in metabolism, apoptosis, cell adhesion, immune response, stress response, and response to endogenous and external stimulus. Hierarchical clustering analyses showed that proteins with similar expression patterns mostly belonged t…
abstract: Haliotis midae is an economically important South African abalone species which has been cultured since the 1990s. H. midae farming constitutes approximately 93% of the country's total marine aquaculture industry. However, the slow growth rate of this animal and the potential for disease outbreaks remain a concern for farmers. Therefore, government and private institutions have joined efforts to investigate ways of enhancing abalone production, to increase abalone growth rates and improve disease resistance. Several studies have shown that probiotic microorganisms can significantly increase the growth rate and disease resistance of H. midae. To date, no comprehensive studies have been conducted to characterise these physiological improvements at the molecular level. Thus, the aim of this study was to evaluate the effect of a probiotic-supplemented diet on the haemocyte proteome in H. midae. Two probiotic-strains, Vibrio midae SY9 and Debaryomuces hansenii AY1, were introduced into H. midae via a kelp-based feed. Changes in the haemocyte proteome were analysed using isobaric tag for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS. A total of 128 haemocyte proteins were identified. Proteins that were found to vary significantly in their expression levels in haemocytes sampled from abalone fed the probiotic-supplemented diet were identified as COP9 signalosome subunit 4, phosphorylase, T-complex protein 1 subunit gamma, V-type proton ATPase subunit B, Rab 1 and Ra-related protein Rab 1A. Differential expression of COP9 signalosome subunit 4 (up-regulated) and Ras-related protein Rab 1A (down-regulated) was confirmed by western blot analysis. Bioinformatics analysis revealed proteins with immune class GO terms that functioned in metabolism, apoptosis, cell adhesion, immune response, stress response, and response to endogenous and external stimulus. Hierarchical clustering analyses showed that proteins with similar expression patterns mostly belonged t…
author: Dias, Valera Lucena
language: eng
date published: 2016
date modified: 2026-07-22T22:22:41Z
provider: University of Cape Town
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Department of Molecular and Cell Biology

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property ID: Handle
value: http://hdl.handle.net/11427/22887