{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/22110"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/22110","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli","abstract":"This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented.","abstract_html":"This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented.","abstract_has_math":false,"creators":["Blatch, Gregory Lloyd"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Woods, David R"],"committee_chairs":[],"committee_members":[],"year":1990,"date_issued":"1990","date_published":"1990","updated_at":"2026-07-22T22:22:42Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/22110","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Woods, David R"]},{"key":"dc:creator","label":"Author","values":["Blatch, Gregory Lloyd"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-10-10T14:56:52Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-10-10T14:56:52Z"]},{"key":"dc:date.issued","label":"Date","values":["1990"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/22110"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Bibliography: pages 135-150."]},{"key":"dc:description.abstract","label":"Abstract","values":["This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented."]},{"key":"dc:title","label":"Title","values":["Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli"]}]}],"canonical_facts":{"dc:contributor.advisor":["Woods, David R"],"dc:creator":["Blatch, Gregory Lloyd"],"dc:date.accessioned":["2016-10-10T14:56:52Z"],"dc:date.available":["2016-10-10T14:56:52Z"],"dc:date.issued":["1990"],"dc:description":["Bibliography: pages 135-150."],"dc:description.abstract":["This dissertation represents a continuation of the research on the sucrose utilization system of the aerobic, collagenolytic, halotolerant, Gram-negative bacterium Vibrio alginolyticus. The V. alginolyticus sucrose utilization system originally cloned into Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented."],"dc:identifier.uri":["http://hdl.handle.net/11427/22110"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:42Z"}