Molecular genetic studies of bacteroides fragilis

Some genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified bacteriocin was a protein with an apparent Mr of 6400-7200, and was relatively heat stable. The action of the bacteriocin resulted in the lysis of sensitive bacteria. As previous reports indicated that a bacteriocin isolated from the BF-1 strain inhibited the action of RNA-polymerase in-vivo and in-vitro, RNA-polymerase was extracted and partially purified from the bacteriocin sensitive B.fragilis BF-2 strain (described in Appendix 5). This enzyme was essentially similar to that described for other Eubacteriales, but was not inhibited in the in vitro assay by the purified bacteriocin described in this thesis.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

1986

Author dc:creator

• Southern, James Arnold

Advisor dc:contributor.advisor

• Woods, David R

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/21909

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/21909