{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/20852"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/20852","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"The impact of myeloid derived suppressor cells on vaccine immunogenicity in South African HIV-infected and uninfected mothers and their infants","abstract":"BACKGROUND: Each year over 4 million infants die from infections, of which many are vaccinepreventable. Young infants respond poorly to vaccines, but the basis of reduced immunity is controversial. We hypothesized that myeloid-derived suppressor cells (MDSC) that might be induced during gestation, would persist at birth leading to active suppression of infant-immune responses. OBJECTIVE: We evaluated the ontogeny of MDSC and the effect of MDSC on vaccine immunogenicity during early life in South African infants and mothers, and in HIVexposed uninfected (HEU) infants and HIV+ mothers. METHODS: HIV-infected and uninfected mothers and their infants were recruited from Khayelitsha, Cape Town and followed-up for one year. In whole PBMC and after MDSC (CD15+) depleted, we measured BCG, Hepatitis B, Tetanus toxoid and Bordetella pertussis vaccine-specific CD4+ T cell proliferation by CFSE and IFN-γ responses using ELISpot assay.","abstract_html":"BACKGROUND: Each year over 4 million infants die from infections, of which many are vaccinepreventable. Young infants respond poorly to vaccines, but the basis of reduced immunity is controversial. We hypothesized that myeloid-derived suppressor cells (MDSC) that might be induced during gestation, would persist at birth leading to active suppression of infant-immune responses. OBJECTIVE: We evaluated the ontogeny of MDSC and the effect of MDSC on vaccine immunogenicity during early life in South African infants and mothers, and in HIVexposed uninfected (HEU) infants and HIV+ mothers. METHODS: HIV-infected and uninfected mothers and their infants were recruited from Khayelitsha, Cape Town and followed-up for one year. In whole PBMC and after MDSC (CD15+) depleted, we measured BCG, Hepatitis B, Tetanus toxoid and Bordetella pertussis vaccine-specific CD4+ T cell proliferation by CFSE and IFN-γ responses using ELISpot assay.","abstract_has_math":false,"creators":["Kidzeru, Elvis Banboye"],"institution":"Division of Immunology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Jaspan, Heather B"],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016","date_published":"2016","updated_at":"2026-07-22T22:22:42Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/20852","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Jaspan, Heather B"]},{"key":"dc:creator","label":"Author","values":["Kidzeru, Elvis Banboye"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-07-27T10:21:42Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-07-27T10:21:42Z"]},{"key":"dc:date.issued","label":"Date","values":["2016"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Immunology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/20852"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["BACKGROUND: Each year over 4 million infants die from infections, of which many are vaccinepreventable. Young infants respond poorly to vaccines, but the basis of reduced immunity is controversial. We hypothesized that myeloid-derived suppressor cells (MDSC) that might be induced during gestation, would persist at birth leading to active suppression of infant-immune responses. OBJECTIVE: We evaluated the ontogeny of MDSC and the effect of MDSC on vaccine immunogenicity during early life in South African infants and mothers, and in HIVexposed uninfected (HEU) infants and HIV+ mothers. METHODS: HIV-infected and uninfected mothers and their infants were recruited from Khayelitsha, Cape Town and followed-up for one year. In whole PBMC and after MDSC (CD15+) depleted, we measured BCG, Hepatitis B, Tetanus toxoid and Bordetella pertussis vaccine-specific CD4+ T cell proliferation by CFSE and IFN-γ responses using ELISpot assay."]},{"key":"dc:title","label":"Title","values":["The impact of myeloid derived suppressor cells on vaccine immunogenicity in South African HIV-infected and uninfected mothers and their infants"]}]}],"canonical_facts":{"dc:contributor.advisor":["Jaspan, Heather B"],"dc:creator":["Kidzeru, Elvis Banboye"],"dc:date.accessioned":["2016-07-27T10:21:42Z"],"dc:date.available":["2016-07-27T10:21:42Z"],"dc:date.issued":["2016"],"dc:description.abstract":["BACKGROUND: Each year over 4 million infants die from infections, of which many are vaccinepreventable. Young infants respond poorly to vaccines, but the basis of reduced immunity is controversial. We hypothesized that myeloid-derived suppressor cells (MDSC) that might be induced during gestation, would persist at birth leading to active suppression of infant-immune responses. OBJECTIVE: We evaluated the ontogeny of MDSC and the effect of MDSC on vaccine immunogenicity during early life in South African infants and mothers, and in HIVexposed uninfected (HEU) infants and HIV+ mothers. METHODS: HIV-infected and uninfected mothers and their infants were recruited from Khayelitsha, Cape Town and followed-up for one year. In whole PBMC and after MDSC (CD15+) depleted, we measured BCG, Hepatitis B, Tetanus toxoid and Bordetella pertussis vaccine-specific CD4+ T cell proliferation by CFSE and IFN-γ responses using ELISpot assay."],"dc:identifier.uri":["http://hdl.handle.net/11427/20852"],"dc:language.iso":["eng"],"dc:publisher.department":["Division of Immunology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["The impact of myeloid derived suppressor cells on vaccine immunogenicity in South African HIV-infected and uninfected mothers and their infants"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:42Z"}