Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study

The presence of calcium (Ca) channels has been investigated in the apical membrane of various segments of rabbit renal tubules and in the basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but were probably of proximal origin. The luminal surfaces of the individual tubule segments were accessed by perfusing the tubule and inserting the patch pipette through the open end or, more frequently, by tearing open the tubule to allow direct access by a patch electrode. Either Ba (90 mM) or Ca (70 mM) was used in the pipettes. Where possible, channel activity was sought in voltage clamped (30 to -60 m V) excised patches. The data were digitized at l kHz, and filtered (200- 500 Hz) by a six-pole Bessel filter.

Grantor dc:publisher.institution

MRC/UCT RU for Exercise and Sport Medicine

Year dc:date.issued

1992

Author dc:creator

• Saunders, James

Advisor dc:contributor.advisor

• Isaacson, Leon

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/17308

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/17308