An electrophoretic method for the isolation of isohistones from the embryo of the sea urchin Parechinus Angulosus

It is the aim of this project to isolate sea urchin embryo histone variants by preparative polyacrylamide gel electrophoresis and to prove me identity of the isolated proteins by amino acid composition and partial sequencing. This serves two purposes, namely, the unequivocal identification of chromosomal proteins characterized only by their electrophoretic mobility as histone variants, and secondly the creation of a micropreparative electrophoretic method. These are the prerequisites for later investigations into the specific roles of histones in the process of differentiation.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

1981

Author dc:creator

• Schwager, Sylva

Advisor dc:contributor.advisor

• Von Holt, Claus

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/15448

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/15448