{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/15447"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/15447","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus","abstract":"The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance.","abstract_html":"The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance.","abstract_has_math":false,"creators":["Botha, Christine Leone"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Kirby, Ralph"],"committee_chairs":[],"committee_members":[],"year":1981,"date_issued":"1981","date_published":"1981","updated_at":"2026-07-22T22:22:38Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/15447","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Kirby, Ralph"]},{"key":"dc:creator","label":"Author","values":["Botha, Christine Leone"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-11-30T08:28:32Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-11-30T08:28:32Z"]},{"key":"dc:date.issued","label":"Date","values":["1981"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/15447"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Bibliography: leaves 89-96."]},{"key":"dc:description.abstract","label":"Abstract","values":["The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance."]},{"key":"dc:title","label":"Title","values":["Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus"]}]}],"canonical_facts":{"dc:contributor.advisor":["Kirby, Ralph"],"dc:creator":["Botha, Christine Leone"],"dc:date.accessioned":["2015-11-30T08:28:32Z"],"dc:date.available":["2015-11-30T08:28:32Z"],"dc:date.issued":["1981"],"dc:description":["Bibliography: leaves 89-96."],"dc:description.abstract":["The Actinomycete organism, Streptomyces alboflavus, produces the antibiotic oxytetracycline. Treatment with curing agents resulted in S. alboflavus losing the ability to produce oxytetracycline. However, the loss of oxytetracyc1ine production was reversible, and non-producing colonies regained the ability to produce oxytetracycline when the curing agent was removed. Therefore the loss of oxytetracyc1ine production was not due to the irreversible loss of genetic material specifying oxytetracycline production, but was possibly due to genetic instability. Two extrachromosomal DNA species were isolated from S. alboflavus, (SAP1 and SAP2). SAP1 was approximately 8 - 10 x 10â ¶ da1tons, and appeared to be linear and heterogenous. SAP2 was 20 - 25 x 10â ¶ daltons and appeared to be covalently closed circular. The functions of SAP1 and SAP2 are unknown, although transformation experiments with SAP1 suggested that it may play a role in the production of an antibiotic-like substance, possibly by acting as a promotor of the genes coding for the antibiotic-like substance."],"dc:identifier.uri":["http://hdl.handle.net/11427/15447"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Generic studies on the oxytetracycline producing organism, Streptomyces alboflavus"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc"]},"updated_at":"2026-07-22T22:22:38Z"}