{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/13965"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/13965","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas","abstract":"Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples.","abstract_html":"Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples.","abstract_has_math":false,"creators":["Rademan, Anina"],"institution":"Division of Anatomical Pathology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Govender, Dhiren"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014","date_published":"2014","updated_at":"2026-07-22T22:22:39Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/13965","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Govender, Dhiren"]},{"key":"dc:creator","label":"Author","values":["Rademan, Anina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-09-15T10:22:33Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-09-15T10:22:33Z"]},{"key":"dc:date.issued","label":"Date","values":["2014"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Anatomical Pathology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc (Med)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/13965"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples."]},{"key":"dc:title","label":"Title","values":["Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas"]}]}],"canonical_facts":{"dc:contributor.advisor":["Govender, Dhiren"],"dc:creator":["Rademan, Anina"],"dc:date.accessioned":["2015-09-15T10:22:33Z"],"dc:date.available":["2015-09-15T10:22:33Z"],"dc:date.issued":["2014"],"dc:description.abstract":["Breast cancer management continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated for their relevance as potential prognostic markers or indicators for treatment. A secondary objective was to evaluate the CISH technique for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained fine needle aspiration (FNA) samples. Tissue blocks from a retrospective series of 93 primary breast carcinoma cases were selected, based on their Her-2 status. Twenty six of these cases received trastuzumab treatment while 67 did not. Expression of Her-2, ER, PI3K, PTEN, p-Akt, BCL2, NFκB, MDM2 and p53 were analysed and compared with various clinicopathological features. The CISH technique was evaluated for its suitability for analysis of Her-2 gene amplification on archived, Papanicolaou-stained FNA samples."],"dc:identifier.uri":["http://hdl.handle.net/11427/13965"],"dc:language.iso":["eng"],"dc:publisher.department":["Division of Anatomical Pathology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc (Med)"]},"updated_at":"2026-07-22T22:22:39Z"}