{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/12618"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/12618","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"Heavy metal interactions with nucleic acid derivatives : a nuclear magnetic resonance study","abstract":"The aqueous solution conformation of SAH and SAM has been investigated using lanthanide probes. SAH at pH 7.0 interacts normally with lanthanide aquo ions and lanthanide-EDTA complexes via coordination at the carboxyl group. The conformation of the homocysteine fragment is found to, be extended but bent back towards the adenine base. SAM does not interact with lanthanide aquo ions in the pH region 2-7. It does form complexes with lanthanide-EDTA species via an ion-pairing mechanism rather than direct coordination. In addition to lanthanide probes, 250 MHz 1H n.m.r. spectra yielded vicinal JHH coupling constants from which the ribose conformation in both SAM and SAH could be more accurately determined.","abstract_html":"The aqueous solution conformation of SAH and SAM has been investigated using lanthanide probes. SAH at pH 7.0 interacts normally with lanthanide aquo ions and lanthanide-EDTA complexes via coordination at the carboxyl group. The conformation of the homocysteine fragment is found to, be extended but bent back towards the adenine base. SAM does not interact with lanthanide aquo ions in the pH region 2-7. It does form complexes with lanthanide-EDTA species via an ion-pairing mechanism rather than direct coordination. In addition to lanthanide probes, 250 MHz 1H n.m.r. spectra yielded vicinal JHH coupling constants from which the ribose conformation in both SAM and SAH could be more accurately determined.","abstract_has_math":false,"creators":["Koch, Klaus R"],"institution":"Department of Chemistry","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Fazakerley, G V"],"committee_chairs":[],"committee_members":[],"year":1979,"date_issued":"1979","date_published":"1979","updated_at":"2026-07-22T22:22:52Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/12618","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Fazakerley, G V"]},{"key":"dc:creator","label":"Author","values":["Koch, Klaus R"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-03-16T10:31:07Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-03-16T10:31:07Z"]},{"key":"dc:date.issued","label":"Date","values":["1979"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Chemistry"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/12618"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Bibliography: p. 181-184."]},{"key":"dc:description.abstract","label":"Abstract","values":["The aqueous solution conformation of SAH and SAM has been investigated using lanthanide probes. SAH at pH 7.0 interacts normally with lanthanide aquo ions and lanthanide-EDTA complexes via coordination at the carboxyl group. The conformation of the homocysteine fragment is found to, be extended but bent back towards the adenine base. SAM does not interact with lanthanide aquo ions in the pH region 2-7. It does form complexes with lanthanide-EDTA species via an ion-pairing mechanism rather than direct coordination. In addition to lanthanide probes, 250 MHz 1H n.m.r. spectra yielded vicinal JHH coupling constants from which the ribose conformation in both SAM and SAH could be more accurately determined."]},{"key":"dc:title","label":"Title","values":["Heavy metal interactions with nucleic acid derivatives : a nuclear magnetic resonance study"]}]}],"canonical_facts":{"dc:contributor.advisor":["Fazakerley, G V"],"dc:creator":["Koch, Klaus R"],"dc:date.accessioned":["2015-03-16T10:31:07Z"],"dc:date.available":["2015-03-16T10:31:07Z"],"dc:date.issued":["1979"],"dc:description":["Bibliography: p. 181-184."],"dc:description.abstract":["The aqueous solution conformation of SAH and SAM has been investigated using lanthanide probes. SAH at pH 7.0 interacts normally with lanthanide aquo ions and lanthanide-EDTA complexes via coordination at the carboxyl group. The conformation of the homocysteine fragment is found to, be extended but bent back towards the adenine base. SAM does not interact with lanthanide aquo ions in the pH region 2-7. It does form complexes with lanthanide-EDTA species via an ion-pairing mechanism rather than direct coordination. In addition to lanthanide probes, 250 MHz 1H n.m.r. spectra yielded vicinal JHH coupling constants from which the ribose conformation in both SAM and SAH could be more accurately determined."],"dc:identifier.uri":["http://hdl.handle.net/11427/12618"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Chemistry"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["Heavy metal interactions with nucleic acid derivatives : a nuclear magnetic resonance study"],"dc:type":["Doctoral Thesis"],"dc:type.qualificationlevel":["Doctoral"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-22T22:22:52Z"}