Process integration in the optimisation of amidase production from recombinant Escherichia coli

This thesis presents the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a tool for minimising the unit operations in the amidase purification train. The EBA process can integrate up to four steps by merging processes involved in centrifugation, microfiltration and initial adsorption into one unit operation.

Grantor dc:publisher.institution

Centre for Bioprocess Engineering Research

Year dc:date.issued

2008

Author dc:creator

• Olaofe, Oluwafemi Ayokunle

Advisor dc:contributor.advisor

• Harrison, STL

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/10857

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/10857