Investigation of XvSap promoters from the resurrection plant, Xerophyta viscosa

The XvPSap1 promoter derived from Xerophyta viscosa has been demonstrated to be stress-inducible during dehydration in transgenic Nicotiana tabacum, black Mexican sweetcorn cells and Zea mays. To improve this promoter, for future applications in crop biotechnology, four shortened promoters, XvSap1D, E, F and G were generated by mutagenesis. The generated promoters had circa 50% reduction in size and contained the 5' proximal and 3' distal regions of the XvPsap1 promoter with the internal region removed. The shortened promoters displayed no significant sequence homology to any other known plant promoter, besides XvPSap1. In addition to the shortened promoters, a newly discovered full length XvPSap2 promoter, showing a 56.41% homology with XvPSap 1 was also assessed in this study.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2012

Author dc:creator

• Ellick, Tamaryn Lorean

Advisor dc:contributor.advisor

• Rafudeen, Mohamed S

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/10452

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/10452