Abstract
dc:description.abstractNature has evolved proteins to form the fundamental machinery of life through controlled assembly. Such protein assembly can span five orders of magnitude in length scale, from subnanometer intramolecular arrangements to the formation of micron- sized compartments. However, our understanding of protein assembly has largely been confined to the structural organisation and pairwise interactions of individual proteins at the nanoscale. In this thesis, I explore protein assembly processes that transcend the molecular scale, emphasising their significance in functional biological processes and therapeutic intervention strategies. Firstly, this thesis establishes a set of methodological advancements capable of addressing current limitations in the study of protein condensation and aggregation, to tackle fundamental biological questions and enable disease biomarker detection. These advancements enable the discovery of previously unexplored emergent protein functions arising from condensation, such as the formation of electrochemical gradients and antimicrobial activity. Furthermore, this thesis advances our grasp of protein assembly specificity by controlling surface chemistry to improve development pipelines of protein therapeutics. Overall, this work expands our understanding of biomolecular function and malfunction, providing tools to decode protein behavior across length scales and guiding future therapeutic intervention strategies.
Degree
thesis:*- Name dc:type.qualificationname
- Doctor of Philosophy (PhD)
- Level dc:type.qualificationlevel
- Doctoral
- Grantor dc:publisher.institution
- University of Cambridge
- Year dc:date.issued
- 2024
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Ausserwoeger, Hannes
- Advisor dc:contributor.advisor
-
- Knowles, Tuomas PJ
Subjects
dc:subject × 5Rights
dc:rightsIdentifiers
dc:identifier.*- DOI dc:identifier.doi
- https://doi.org/10.17863/CAM.116851
- OAI identifier oai:identifier
- oai:www.repository.cam.ac.uk:1810/381784