{"id":{"repo_id":"cambridge","oai_identifier":"oai:www.repository.cam.ac.uk:1810/369357"},"canonical_url":"https://search.dev.ndltd.org/etd/cambridge/oai:www.repository.cam.ac.uk:1810/369357","repository":{"repo_id":"cambridge","name":"Cambridge University","base_url":"https://api.repository.cam.ac.uk/server/oai/request"},"display":{"title":"Evolutionary and functional study of plant cell wall polysaccharide glucomannan","abstract":"My PhD thesis focused on the cell wall polysaccharide mannan; I investigated the function, evolution, and mutant phenotypes of its synthase. The main chapter consists of three independent parts. Chapter2 addresses the question, “In which group of evolutionary stages does β-GGM (β-galactoglucomannan) exist?”. The results suggest that β-GGM is likely to be specific to dicots and that the key event of acquisition of MBGT (mannan bgalactosyltransferase) activity may have occurred in this group. Chapter3 addressed the following question: “Was MBGT acquired convergently in Asterids and Rosids?” The results show that MBGT in Rosids is present in GT47A-VII (Glycosyltransferase family 47A subclade VII), whereas that in Asterids is present in GT47A-III, suggesting convergent evolution from different xyloglucan galactosyltransferases. Chapter4 addressed the question, “What is the role of CSLD (cellulose synthase-like D) glucan?\" Although we could not clarify a role for CSLD glucans, we found that constitutive immune response occurs in the *csld5* mutant. Furthermore, we found that lignin deposition induced by a transcription factor MYB15 (myeloblastosis family 15) enhances pathogen resistance in *csld5*. These results not only demonstrate the diversity of mannan-modifying enzymes, but also highlight the importance of their physiological roles, which plants have acquired throughout their evolutionary history. Furthermore, they show that CSLD glucans, polysaccharides related to mannan, are not just minor structures but also have a role in the context of adaptation and immunity.","abstract_html":"My PhD thesis focused on the cell wall polysaccharide mannan; I investigated the function, evolution, and mutant phenotypes of its synthase. The main chapter consists of three independent parts. Chapter2 addresses the question, “In which group of evolutionary stages does β-GGM (β-galactoglucomannan) exist?”. The results suggest that β-GGM is likely to be specific to dicots and that the key event of acquisition of MBGT (mannan bgalactosyltransferase) activity may have occurred in this group. Chapter3 addressed the following question: “Was MBGT acquired convergently in Asterids and Rosids?” The results show that MBGT in Rosids is present in GT47A-VII (Glycosyltransferase family 47A subclade VII), whereas that in Asterids is present in GT47A-III, suggesting convergent evolution from different xyloglucan galactosyltransferases. Chapter4 addressed the question, “What is the role of CSLD (cellulose synthase-like D) glucan?" Although we could not clarify a role for CSLD glucans, we found that constitutive immune response occurs in the *csld5* mutant. Furthermore, we found that lignin deposition induced by a transcription factor MYB15 (myeloblastosis family 15) enhances pathogen resistance in *csld5*. These results not only demonstrate the diversity of mannan-modifying enzymes, but also highlight the importance of their physiological roles, which plants have acquired throughout their evolutionary history. Furthermore, they show that CSLD glucans, polysaccharides related to mannan, are not just minor structures but also have a role in the context of adaptation and immunity.","abstract_has_math":false,"creators":["Ishida, Konan"],"institution":"University of Cambridge","degree_name":"Doctor of Philosophy (PhD)","degree_level":"Doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Dupree, Paul"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-01-29","date_published":"2024-01-29","updated_at":"2026-07-22T22:24:25Z","subjects":["Evolution","Glycosyltransferase","Plant cell wall","Plant physiology"],"languages":["eng"],"rights":[],"rights_urls":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/2096b5fe-baf4-4a81-863d-fee7652e484d/download","https://www.rioxx.net/licenses/all-rights-reserved/"],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.17863/CAM.109202","outbound_label":"DOI","outbound_source":"dc:identifier.doi"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Dupree, Paul"]},{"key":"dc:creator","label":"Author","values":["Ishida, Konan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2024-01-29"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cambridge"]},{"key":"dc:relation.isreferencedby.uri","label":"Dc Relation Isreferencedby URI","values":["https://www.repository.cam.ac.uk/handle/1810/369357"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Evolution","Glycosyltransferase","Plant cell wall","Plant physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/2096b5fe-baf4-4a81-863d-fee7652e484d/download","https://www.rioxx.net/licenses/all-rights-reserved/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.17863/CAM.109202"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/096ddd21-04ad-4f18-a038-e91a5d8dbe48/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["My PhD thesis focused on the cell wall polysaccharide mannan; I investigated the function, evolution, and mutant phenotypes of its synthase. 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Furthermore, we found that lignin deposition induced by a transcription factor MYB15 (myeloblastosis family 15) enhances pathogen resistance in *csld5*. These results not only demonstrate the diversity of mannan-modifying enzymes, but also highlight the importance of their physiological roles, which plants have acquired throughout their evolutionary history. 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The results suggest that β-GGM is likely to be specific to dicots and that the key event of acquisition of MBGT (mannan bgalactosyltransferase) activity may have occurred in this group. Chapter3 addressed the following question: “Was MBGT acquired convergently in Asterids and Rosids?” The results show that MBGT in Rosids is present in GT47A-VII (Glycosyltransferase family 47A subclade VII), whereas that in Asterids is present in GT47A-III, suggesting convergent evolution from different xyloglucan galactosyltransferases. Chapter4 addressed the question, “What is the role of CSLD (cellulose synthase-like D) glucan?\" Although we could not clarify a role for CSLD glucans, we found that constitutive immune response occurs in the *csld5* mutant. Furthermore, we found that lignin deposition induced by a transcription factor MYB15 (myeloblastosis family 15) enhances pathogen resistance in *csld5*. 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