{"id":{"repo_id":"cambridge","oai_identifier":"oai:www.repository.cam.ac.uk:1810/365177"},"canonical_url":"https://search.dev.ndltd.org/etd/cambridge/oai:www.repository.cam.ac.uk:1810/365177","repository":{"repo_id":"cambridge","name":"Cambridge University","base_url":"https://api.repository.cam.ac.uk/server/oai/request"},"display":{"title":"A Single Cell Approach to TCR Signal Strength","abstract":"Cytotoxic T lymphocytes (CTLs) play a key role in the cell-mediated immune response against virally-infected and tumourigenic cells, killing their targets through the release of cytolytic granules. T cell receptor (TCR) recognition of foreign peptides presented by Class I MHC molecules stimulates naïve CD8+ T cell differentiation to effector cells and triggers the cytolytic activity of effector CTLs. Signal transduction downstream of the TCR is a highly diverse and coordinated network of post-translational protein modifications that ultimately drive transcriptional, translational, metabolic and cytoskeletal changes in the cell. How cytotoxic T cells coordinate their molecular machinery in response to strong versus weak stimuli remains unclear. Utilising single-cell methods including mass cytometry and single-cell RNA-sequencing, this study demonstrates how naïve and restimulated cytotoxic T cells coordinate their responses against peptides of differing stimulation strengths.","abstract_html":"Cytotoxic T lymphocytes (CTLs) play a key role in the cell-mediated immune response against virally-infected and tumourigenic cells, killing their targets through the release of cytolytic granules. T cell receptor (TCR) recognition of foreign peptides presented by Class I MHC molecules stimulates naïve CD8+ T cell differentiation to effector cells and triggers the cytolytic activity of effector CTLs. Signal transduction downstream of the TCR is a highly diverse and coordinated network of post-translational protein modifications that ultimately drive transcriptional, translational, metabolic and cytoskeletal changes in the cell. How cytotoxic T cells coordinate their molecular machinery in response to strong versus weak stimuli remains unclear. Utilising single-cell methods including mass cytometry and single-cell RNA-sequencing, this study demonstrates how naïve and restimulated cytotoxic T cells coordinate their responses against peptides of differing stimulation strengths.","abstract_has_math":false,"creators":["Ma, Claire"],"institution":"University of Cambridge","degree_name":"Doctor of Philosophy (PhD)","degree_level":"Doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Griffiths, Gillian"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-01-01","date_published":"2024-01-01","updated_at":"2026-07-22T22:24:03Z","subjects":["CTL","Cytof","Cytotoxic T cells","mass cytometry","signal strength","signalling","single cell","TCR"],"languages":["eng"],"rights":[],"rights_urls":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/f9954baa-dff3-438d-9095-6871deb0a5bb/download","http://purl.org/NET/rdflicense/allrightsreserved"],"identifier_entries":[{"key":"dc:creator.authoridentifier","label":"Author Identifier","values":["0000000242447535"],"render_values":[{"text":"0000-0002-4244-7535","href":"https://orcid.org/0000-0002-4244-7535","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.17863/CAM.106571","outbound_label":"DOI","outbound_source":"dc:identifier.doi"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Griffiths, Gillian"]},{"key":"dc:creator","label":"Author","values":["Ma, Claire"]},{"key":"dc:creator.authoridentifier","label":"Author Identifier","values":["0000000242447535"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2024-01-01"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cambridge"]},{"key":"dc:relation.isreferencedby.uri","label":"Dc Relation Isreferencedby URI","values":["https://www.repository.cam.ac.uk/handle/1810/365177"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["CTL","Cytof","Cytotoxic T cells","mass cytometry","signal strength","signalling","single cell","TCR"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/f9954baa-dff3-438d-9095-6871deb0a5bb/download","http://purl.org/NET/rdflicense/allrightsreserved"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.17863/CAM.106571"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/e9d2141e-de1d-41c6-91a2-1397e2f8f50b/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Cytotoxic T lymphocytes (CTLs) play a key role in the cell-mediated immune response against virally-infected and tumourigenic cells, killing their targets through the release of cytolytic granules. 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