Cancer Cartography: Mapping Cancer Evolution in Tissue Space
type: CreativeWork
description: While somatic evolution is widely accepted as fundamental to cancer development, significant gaps in understanding persist. These gaps concern the role of cellular interactions within the tumour microenvironment (TME) and the impact of spatial constraints on cancer evolution. Emerging spatial omics technologies offer the potential to address these gaps, although their application in spatial genomics remains limited. This is particularly crucial because genetic alterations not only drive cancer evolution but also serve as an archive of its history. To address this research gap, this thesis aims to develop computational methods for analysing spatial genomics data, specifically on data generated by base-specific in situ sequencing (BaSISS), a method that enables high-resolution mapping of diverse somatic mutations across large tumour tissue sections. Bayesian algorithms designed in the study generate quantitative clonal maps, effectively tracing cancer evolution in tissue space while accommodating multiple forms of biological and technical variability. Despite inherent assumptions, the algorithm demonstrates robustness and quantitative accuracy. Complementary data types contribute to the findings by applying the Bayesian model to two multifocal breast cancers at different stages of progression. Integration of histology, immunohistochemistry (IHC), and targeted in situ gene expression allows for the phenotypic characterisation of clones in distinct microanatomical niches. Subsequent analyses across various stages of breast cancer, including carcinoma in situ, invasive cancer, and lymph node metastasis reveal clone-specific variations in proliferation, morphology, stroma, hypoxia, and immune microenvironments. In one instance involving ductal carcinoma in situ, polyclonal neoplastic expansions manifest on a macroscopic scale but remain segregated within microanatomical structures. In summary, this thesis establishes a robust computational framework for extracting clonal …
abstract: While somatic evolution is widely accepted as fundamental to cancer development, significant gaps in understanding persist. These gaps concern the role of cellular interactions within the tumour microenvironment (TME) and the impact of spatial constraints on cancer evolution. Emerging spatial omics technologies offer the potential to address these gaps, although their application in spatial genomics remains limited. This is particularly crucial because genetic alterations not only drive cancer evolution but also serve as an archive of its history. To address this research gap, this thesis aims to develop computational methods for analysing spatial genomics data, specifically on data generated by base-specific in situ sequencing (BaSISS), a method that enables high-resolution mapping of diverse somatic mutations across large tumour tissue sections. Bayesian algorithms designed in the study generate quantitative clonal maps, effectively tracing cancer evolution in tissue space while accommodating multiple forms of biological and technical variability. Despite inherent assumptions, the algorithm demonstrates robustness and quantitative accuracy. Complementary data types contribute to the findings by applying the Bayesian model to two multifocal breast cancers at different stages of progression. Integration of histology, immunohistochemistry (IHC), and targeted in situ gene expression allows for the phenotypic characterisation of clones in distinct microanatomical niches. Subsequent analyses across various stages of breast cancer, including carcinoma in situ, invasive cancer, and lymph node metastasis reveal clone-specific variations in proliferation, morphology, stroma, hypoxia, and immune microenvironments. In one instance involving ductal carcinoma in situ, polyclonal neoplastic expansions manifest on a macroscopic scale but remain segregated within microanatomical structures. In summary, this thesis establishes a robust computational framework for extracting clonal …
author: Lomakin, Artem
language: eng
keywords: Cancer Genomics, Computational Biology, Spatial Genomics
license: https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/3de460eb-1c97-481f-9e1b-409b0e9e9f91/download
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2023-09
date modified: 2026-07-22T22:24:10Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.106003