Single-cell Protein-Transcriptome Atlas of Haematopoiesis across the Human Lifespan
type: CreativeWork
description: The haematopoietic system is responsible for the continuous production of stem and progenitor cells, which divide and mature to produce a wide variety of specialised circulating blood cells. Proper regulation of lineage decisions is key to ensure the right balance between the generation of differentiated cells and the maintenance of the haematopoietic stem cell compartment. A dysregulation in this balance can lead to blood-related diseases. Throughout an individual's life, human haematopoiesis demonstrates significant variations, likely in response to changing demands and physiological needs. Moreover, age-related alterations in the blood system are believed to impede its functionality. Both transcriptomic and proteomic regulation play crucial roles in maintaining the haematopoietic balance. Interestingly, many classical haematopoietic populations showcase heterogeneity in both expression and function. Studying genes and cell surface proteins at the single-cell level using a combination of scRNA-seq and index sorting has proven valuable for inferring individual cellular states and identifying subpopulations for further research. Nevertheless, traditional methods that measure both transcripts and proteins from single cells via index sorting/FACS often have scale limitations. Typically, they can provide data on only a limited number of genes and proteins when used in parallel. These constraints make it difficult to capture the full complexity of cellular states and do not enable tissue scale analysis. We take advantage of the method Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), which enables the simultaneous combination of highly multiplexed cell surface protein marker detection with unbiased transcriptome profiling for thousands of single cells, allowing more detailed characterisation of cellular phenotypes than transcriptome measurements alone. To investigate age-related changes, cells can be gathered from tissues representing various st…
abstract: The haematopoietic system is responsible for the continuous production of stem and progenitor cells, which divide and mature to produce a wide variety of specialised circulating blood cells. Proper regulation of lineage decisions is key to ensure the right balance between the generation of differentiated cells and the maintenance of the haematopoietic stem cell compartment. A dysregulation in this balance can lead to blood-related diseases. Throughout an individual's life, human haematopoiesis demonstrates significant variations, likely in response to changing demands and physiological needs. Moreover, age-related alterations in the blood system are believed to impede its functionality. Both transcriptomic and proteomic regulation play crucial roles in maintaining the haematopoietic balance. Interestingly, many classical haematopoietic populations showcase heterogeneity in both expression and function. Studying genes and cell surface proteins at the single-cell level using a combination of scRNA-seq and index sorting has proven valuable for inferring individual cellular states and identifying subpopulations for further research. Nevertheless, traditional methods that measure both transcripts and proteins from single cells via index sorting/FACS often have scale limitations. Typically, they can provide data on only a limited number of genes and proteins when used in parallel. These constraints make it difficult to capture the full complexity of cellular states and do not enable tissue scale analysis. We take advantage of the method Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), which enables the simultaneous combination of highly multiplexed cell surface protein marker detection with unbiased transcriptome profiling for thousands of single cells, allowing more detailed characterisation of cellular phenotypes than transcriptome measurements alone. To investigate age-related changes, cells can be gathered from tissues representing various st…
author: Quiroga Londoño, Mariana
language: eng
keywords: Adult bone marrow, CITE-seq, Cord blood, Extramedullary haematopoiesis, Fetal haematopoiesis, Haematopoiesis, Haematopoietic stem and progenitor cells, Human, Human Cell Atlas, Paediatric bone marrow, Proteomics, Single-cell multi-omics, Transcriptomics
license: https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/dfe7d389-1f0f-4b1e-8aca-85ca9f0267f6/download
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2023-02-10
date modified: 2026-07-22T22:24:23Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.105875