Unravelling the roles of the Mck1 kinase in metabolic reprogramming and the stress response
type: CreativeWork
description: During the transition into stationary phase, S. cerevisiae cells acquire a set of characteristics that are typical of G0 cells, which include upregulation of the starvation- specific gene expression program, enhanced resistance to environmental stressors, the accumulation of storage carbohydrates, the thickening of the cell wall and ultimately the ability to maintain long-term survival. Previous work in the lab revealed that the Mck1 kinase, one of the four GSK3 homologues in Saccharomyces cerevisiae, is a key regulator of stationary phase entry. Further study indicated that Mck1 promotes UDPG synthesis during the transition to the stationary phase to enable cell wall thickening as well as the accumulation of storage carbohydrates. In this Project, I intended to find how the Mck1 kinase regulates metabolic reprogramming and the stress response in response to glucose depletion. In the first result chapter (chapter 3), the functional conservation between the budding S. cerevisiae Mck1 and those from the pathogenic fungi was initially demonstrated. Following-up transcriptome studies revealed that Mck1 may promote metabolic reprogramming and the starvation-induced stress response by activating two regulons: the Cat8- and Adr1-mediated gene expression program promoting the TCA cycle and gluconeogenesis, and the Msn2/4- and /Gis1-dependent stress response. Transcriptome and genetic analyses also suggested that Mck1 regulates metabolic reprogramming and the stress response independent of Mpk1/Slt2, the MAP kinase of the cell wall integrity (CWI) pathway. 5 In the second result chapter (chapter 4), Mck1-regulated proteome changes in response to glucose starvation were revealed. The proteome analysis indicated that Mck1 promotes the expression of the Cat8-/Adr1- and the Msn2/4/Gis1-dependent regulons at the protein levels, supporting that Mck1 regulates metabolic reprogramming and the stress response program mainly at the transcriptional level. The levels of a number of prot…
abstract: During the transition into stationary phase, S. cerevisiae cells acquire a set of characteristics that are typical of G0 cells, which include upregulation of the starvation- specific gene expression program, enhanced resistance to environmental stressors, the accumulation of storage carbohydrates, the thickening of the cell wall and ultimately the ability to maintain long-term survival. Previous work in the lab revealed that the Mck1 kinase, one of the four GSK3 homologues in Saccharomyces cerevisiae, is a key regulator of stationary phase entry. Further study indicated that Mck1 promotes UDPG synthesis during the transition to the stationary phase to enable cell wall thickening as well as the accumulation of storage carbohydrates. In this Project, I intended to find how the Mck1 kinase regulates metabolic reprogramming and the stress response in response to glucose depletion. In the first result chapter (chapter 3), the functional conservation between the budding S. cerevisiae Mck1 and those from the pathogenic fungi was initially demonstrated. Following-up transcriptome studies revealed that Mck1 may promote metabolic reprogramming and the starvation-induced stress response by activating two regulons: the Cat8- and Adr1-mediated gene expression program promoting the TCA cycle and gluconeogenesis, and the Msn2/4- and /Gis1-dependent stress response. Transcriptome and genetic analyses also suggested that Mck1 regulates metabolic reprogramming and the stress response independent of Mpk1/Slt2, the MAP kinase of the cell wall integrity (CWI) pathway. 5 In the second result chapter (chapter 4), Mck1-regulated proteome changes in response to glucose starvation were revealed. The proteome analysis indicated that Mck1 promotes the expression of the Cat8-/Adr1- and the Msn2/4/Gis1-dependent regulons at the protein levels, supporting that Mck1 regulates metabolic reprogramming and the stress response program mainly at the transcriptional level. The levels of a number of prot…
author: Zhang, Fan
language: eng
keywords: Mck1, Yeast
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2022-11-30
date modified: 2026-07-22T22:24:08Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.94433