Virus-induced effects on aphid-host interactions in Solanaceous plants
type: CreativeWork
description: Cucumber mosaic virus (CMV) has three genomic RNA segments, RNAs 1, 2 and 3, that encode five proteins. In some hosts, CMV induces biochemical or physiological changes that may influence virus transmission by aphids. In Arabidopsis thaliana ecotype Col-0 and cucurbits, CMV diminishes host suitability for aphids, inhibiting prolonged feeding and encouraging migration of viruliferous aphids from infected plants to healthy plants. In tomato (Solanum lycopersicum), a model species and economically important crop, viral modification of vector-host interactions for non-persistently transmitted viruses is understudied. I investigated CMV-induced effects on settling preference of two aphid species (Myzus persicae, a generalist, and Macrosiphum euphorbiae, a Solanaceous specialist) at 3, 9 and 21 days post-inoculation (dpi) and at 1 and 24 h following aphid release, using aphid free choice and trapping assays in light and dark conditions and under light of varying wavelengths. Further experiments were carried out to examine whether specific CMV viral proteins control aphid-tomato interactions. Finally, the role of plant signalling pathways in CMV-aphid interactions in tomato and tobacco was investigated. In tomato, the effects of Fny-CMV infection on aphid settling varied as disease progressed. While pre-symptomatic (up to 3 dpi), infected tomato plants were more preferred by Myzus persicae than by Macrosiphum euphorbiae. As symptoms became apparent, between 9 and 21 dpi, more aphids settled on mock-inoculated plants than on virus-infected plants. In trapping assays in the dark, aphids did not prefer either type of plant, despite virus-infected plants emitting greater quantities of volatile organic compounds in the dark than in the light. In olfactometry assays, in which visual and contact cues were not available, aphids of both species were more attracted by odours emitted by virus-infected plants than to those of mock-inoculated plants. Thus, CMV stimulates multiple cues, …
abstract: Cucumber mosaic virus (CMV) has three genomic RNA segments, RNAs 1, 2 and 3, that encode five proteins. In some hosts, CMV induces biochemical or physiological changes that may influence virus transmission by aphids. In Arabidopsis thaliana ecotype Col-0 and cucurbits, CMV diminishes host suitability for aphids, inhibiting prolonged feeding and encouraging migration of viruliferous aphids from infected plants to healthy plants. In tomato (Solanum lycopersicum), a model species and economically important crop, viral modification of vector-host interactions for non-persistently transmitted viruses is understudied. I investigated CMV-induced effects on settling preference of two aphid species (Myzus persicae, a generalist, and Macrosiphum euphorbiae, a Solanaceous specialist) at 3, 9 and 21 days post-inoculation (dpi) and at 1 and 24 h following aphid release, using aphid free choice and trapping assays in light and dark conditions and under light of varying wavelengths. Further experiments were carried out to examine whether specific CMV viral proteins control aphid-tomato interactions. Finally, the role of plant signalling pathways in CMV-aphid interactions in tomato and tobacco was investigated. In tomato, the effects of Fny-CMV infection on aphid settling varied as disease progressed. While pre-symptomatic (up to 3 dpi), infected tomato plants were more preferred by Myzus persicae than by Macrosiphum euphorbiae. As symptoms became apparent, between 9 and 21 dpi, more aphids settled on mock-inoculated plants than on virus-infected plants. In trapping assays in the dark, aphids did not prefer either type of plant, despite virus-infected plants emitting greater quantities of volatile organic compounds in the dark than in the light. In olfactometry assays, in which visual and contact cues were not available, aphids of both species were more attracted by odours emitted by virus-infected plants than to those of mock-inoculated plants. Thus, CMV stimulates multiple cues, …
author: Arinaitwe, Warren
language: eng
keywords: Cucumber mosaic virus, aphids, Myzus persicae, Macrosiphum euphorbiae, tomato
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2021-08
date modified: 2026-07-22T22:24:01Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.81578