Structural and Spatiotemporal Organisation of Bax and Apaf1 during Apoptosis
type: CreativeWork
description: In this thesis, I present insights into the structural, spatiotemporal and functional characteristics of two essential steps of apoptosis, Bax cluster formation and Apaf1 activation, using a combination of biochemical and microscopy approaches. I sought to better understand the contribution of spatial and structural protein organisation within cells towards the efficient promotion of apoptosis, an essential cellular pathway. During apoptosis, Bax oligomerises on the outer mitochondrial membrane promoting mitochondrial ruptures of several hundred nanometres wide and a complete remodelling of the mitochondrion architecture. Using cryo-electron tomography, Bax clusters were shown to form distinct cellular environments organised in a meshwork similar to a sponge-like structure that might be bound to the ruptured outer mitochondrial membrane. This suggests that Bax may sequester components of the membrane into the cytosol. In this thesis, a protein-proximity biotinylation assay in living cells (BioID/TurboID) is presented, which allowed the identification of Bax cluster interactor proteins such as the fission proteins DRP1 and MFF and the mitochondrial matrix chaperone TRAP1. This demonstrates that Bax clusters might sequester various proteins in addition to membrane pieces, possibly favouring the course of apoptosis. Furthermore, correlative light and electron microscopy of resin embedded DRP1KO cells suggest a crucial role for DRP1 in forming Bax clusters and mitochondrial ruptures during apoptosis. Bax activity leads to the release of Cytochrome c from mitochondrial cristae, which interacts with the cytoplasmic protein Apaf1, leading to the formation of a heptameric caspase activation platform called the apoptosome. Here, using fluorescence live-microscopy, it is discovered that Apaf1 forms multiple cytosolic foci during apoptosis which do not co-localise with Bax clusters. Apaf1 foci formation is triggered by the availability of Cytochrome c in the cytosol. Moreover,…
abstract: In this thesis, I present insights into the structural, spatiotemporal and functional characteristics of two essential steps of apoptosis, Bax cluster formation and Apaf1 activation, using a combination of biochemical and microscopy approaches. I sought to better understand the contribution of spatial and structural protein organisation within cells towards the efficient promotion of apoptosis, an essential cellular pathway. During apoptosis, Bax oligomerises on the outer mitochondrial membrane promoting mitochondrial ruptures of several hundred nanometres wide and a complete remodelling of the mitochondrion architecture. Using cryo-electron tomography, Bax clusters were shown to form distinct cellular environments organised in a meshwork similar to a sponge-like structure that might be bound to the ruptured outer mitochondrial membrane. This suggests that Bax may sequester components of the membrane into the cytosol. In this thesis, a protein-proximity biotinylation assay in living cells (BioID/TurboID) is presented, which allowed the identification of Bax cluster interactor proteins such as the fission proteins DRP1 and MFF and the mitochondrial matrix chaperone TRAP1. This demonstrates that Bax clusters might sequester various proteins in addition to membrane pieces, possibly favouring the course of apoptosis. Furthermore, correlative light and electron microscopy of resin embedded DRP1KO cells suggest a crucial role for DRP1 in forming Bax clusters and mitochondrial ruptures during apoptosis. Bax activity leads to the release of Cytochrome c from mitochondrial cristae, which interacts with the cytoplasmic protein Apaf1, leading to the formation of a heptameric caspase activation platform called the apoptosome. Here, using fluorescence live-microscopy, it is discovered that Apaf1 forms multiple cytosolic foci during apoptosis which do not co-localise with Bax clusters. Apaf1 foci formation is triggered by the availability of Cytochrome c in the cytosol. Moreover,…
author: Borgeaud, Alicia
language: eng
keywords: Apoptosis, Apaf1, Bax, Electron microscopy, BioID
license: https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/07fe2138-96ee-4c06-880e-13b4c460e4db/download
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2021-03-31
date modified: 2026-07-22T22:24:21Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.77340