Molecular interactions of plant cell wall polymers
type: CreativeWork
description: Specialised plant cells produce thickened cell walls, called secondary cell walls comprised of lignocellulose. The main polymers in lignocellulose are cellulose, xylan, galactoglucomannan and lignin. Lignocellulose forms the majority of biomass on the planet and its utilization for construction, energy production, materials and pharmaceuticals may be important for a more sustainable future. For each of these applications, the interactions between the polymers in secondary cell walls are important. Previously, it was proposed that glucuronic acid side chains on xylan form ester bonds with lignin, and that this cross-linking might be important for cell wall properties. These bonds have been hypothesized to form if the glucuronic acid substitutions of xylan participate in lignin polymerization reactions and thereby cross-link xylan and lignin. Supporting this potentially important role in cell wall cross-linking, previous investigations in the model plant Arabidopsis showed that the glucuronic acid branches of xylan are crucial to the recalcitrance of lignocellulose to enzymatic digestion. In this thesis the molecular basis of this change in recalcitrance was investigated. The gux1 gux2 mutant, which lacks glucuronic acid in secondary cell walls, was found to be more accessible to hydrolytic enzymes, likely due to an increase in the porosity of the cell wall. Investigations with solid-state NMR revealed a reduction in the interactions between lignin and xylan in the mutant plants. Specific lignin synthesis mutants, which have altered lignification chemistry preventing the xylan-lignin cross-linking, were shown to share enzyme accessibility and lignin-xylan interaction phenotypes with the gux1 gux2 mutant. The presence of ester bonds between lignin and xylan was investigated and introduction of novel xylan-lignin cross-links was attempted. Solid-state NMR was used to extend our understanding of the interactions between the cell wall polymers, in industrially relevant co…
abstract: Specialised plant cells produce thickened cell walls, called secondary cell walls comprised of lignocellulose. The main polymers in lignocellulose are cellulose, xylan, galactoglucomannan and lignin. Lignocellulose forms the majority of biomass on the planet and its utilization for construction, energy production, materials and pharmaceuticals may be important for a more sustainable future. For each of these applications, the interactions between the polymers in secondary cell walls are important. Previously, it was proposed that glucuronic acid side chains on xylan form ester bonds with lignin, and that this cross-linking might be important for cell wall properties. These bonds have been hypothesized to form if the glucuronic acid substitutions of xylan participate in lignin polymerization reactions and thereby cross-link xylan and lignin. Supporting this potentially important role in cell wall cross-linking, previous investigations in the model plant Arabidopsis showed that the glucuronic acid branches of xylan are crucial to the recalcitrance of lignocellulose to enzymatic digestion. In this thesis the molecular basis of this change in recalcitrance was investigated. The gux1 gux2 mutant, which lacks glucuronic acid in secondary cell walls, was found to be more accessible to hydrolytic enzymes, likely due to an increase in the porosity of the cell wall. Investigations with solid-state NMR revealed a reduction in the interactions between lignin and xylan in the mutant plants. Specific lignin synthesis mutants, which have altered lignification chemistry preventing the xylan-lignin cross-linking, were shown to share enzyme accessibility and lignin-xylan interaction phenotypes with the gux1 gux2 mutant. The presence of ester bonds between lignin and xylan was investigated and introduction of novel xylan-lignin cross-links was attempted. Solid-state NMR was used to extend our understanding of the interactions between the cell wall polymers, in industrially relevant co…
author: Terrett, Oliver
language: eng
keywords: plant cell wall, xylan
license: https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/86a386fc-0399-49a1-b6d2-41b1bf2f67c7/download
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2020-07
date modified: 2026-07-22T22:24:20Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.54848