Examination of ex-vivo lung perfusion in porcine model
type: CreativeWork
description: Lung transplantation is a lifesaving therapeutic option for a variety of endstage cardiopulmonary conditions. There is a critical mismatch between the availability of suitable lungs and the number of patients awaiting a lung transplant. Ex-vivo lung perfusion (EVLP) is an established technique that may potentially allow more donor lungs to be reconditioned and provide waiting list patients with a better opportunity for transplantation. During EVLP, lungs are connected to a circuit similar to a cardiopulmonary bypass circuit and are perfused and ventilated under normothaermic conditions. In this thesis I have validated a donor after circulatory death (DCD) large animal model, by using porcine lungs initially from local abattoirs followed by change to a more suitable lungs source. As part of the experimental validation, I have shown that there is an initial bacterial load within the perfusate prior starting EVLP, which improved or disappeared during the procedure. I have characterised the perfusate solution and have demonstrated equivalence of alternative perfusate solutions to the current best practice; I have described the inflammatory profiles of the lungs and studied the impact of novel drug and cell therapies on these lungs. In the first phase I have compared cellular and acellular solutions currently used in practice, aiding in the decision of which solution would be most appropriate for my subsequent experiments. By comparing physiological, immunological, ultrastructural parameters, I have found improved but comparable trends in the blood-based solutions. Due to the cost effectiveness and physiological effects on lungs, I have decided to use the Papworth blood-based perfusate out of the three compared solutions. Lung inflammation and rejection processes are interlinked during lung transplantation. In order to characterise the occurring inflammatory processes, I have quantified the cytokine profile in the airway and perfusate; determined the leukocytes trapped i…
abstract: Lung transplantation is a lifesaving therapeutic option for a variety of endstage cardiopulmonary conditions. There is a critical mismatch between the availability of suitable lungs and the number of patients awaiting a lung transplant. Ex-vivo lung perfusion (EVLP) is an established technique that may potentially allow more donor lungs to be reconditioned and provide waiting list patients with a better opportunity for transplantation. During EVLP, lungs are connected to a circuit similar to a cardiopulmonary bypass circuit and are perfused and ventilated under normothaermic conditions. In this thesis I have validated a donor after circulatory death (DCD) large animal model, by using porcine lungs initially from local abattoirs followed by change to a more suitable lungs source. As part of the experimental validation, I have shown that there is an initial bacterial load within the perfusate prior starting EVLP, which improved or disappeared during the procedure. I have characterised the perfusate solution and have demonstrated equivalence of alternative perfusate solutions to the current best practice; I have described the inflammatory profiles of the lungs and studied the impact of novel drug and cell therapies on these lungs. In the first phase I have compared cellular and acellular solutions currently used in practice, aiding in the decision of which solution would be most appropriate for my subsequent experiments. By comparing physiological, immunological, ultrastructural parameters, I have found improved but comparable trends in the blood-based solutions. Due to the cost effectiveness and physiological effects on lungs, I have decided to use the Papworth blood-based perfusate out of the three compared solutions. Lung inflammation and rejection processes are interlinked during lung transplantation. In order to characterise the occurring inflammatory processes, I have quantified the cytokine profile in the airway and perfusate; determined the leukocytes trapped i…
author: Roman, Marius Andrei
language: eng
license: https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/958aada2-95e6-4c6f-aded-d3f01ebe3e4b/download
license: https://www.rioxx.net/licenses/all-rights-reserved/
date published: 2016-10-14
date modified: 2026-07-22T22:24:10Z
provider: Cambridge University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Cambridge

PropertyValue
property ID: DOI
value: 10.17863/CAM.34272