{"id":{"repo_id":"cambridge","oai_identifier":"oai:www.repository.cam.ac.uk:1810/245124"},"canonical_url":"https://search.dev.ndltd.org/etd/cambridge/oai:www.repository.cam.ac.uk:1810/245124","repository":{"repo_id":"cambridge","name":"Cambridge University","base_url":"https://api.repository.cam.ac.uk/server/oai/request"},"display":{"title":"Development of micro analytical devices","abstract":"This thesis describes the design and development of novel micro analytical devices for application in on-line process analytics. The work describes the design, development, numerical simulation and application of these devices for two specific cases: (i) electrochemical detection of bio(chemical) species at micro-scale and (ii) separation and purification of biological reagents using immobilised metal affinity chromatography at micro-litre scale. Chapter 1 provides a general overview and background to the field of process analytics, microreactors and theory related to the mass transfer inside the electrochemical microfluidic devices and meso-chromatography columns. Chapter 2 provides an overview of microfabrication methods and the numerical simulations employed for the development of micro analytical devices used in this thesis. Chapter 3 describes an experimental voltammetric study of enzyme cofactors in batch and hydrodynamic systems and also provides a numerical investigation of mass transfer over electrodes inside microreactors. Chapter 4 investigates the effect of hydrodynamic focusing within a microfluidic device in detail, using experimental and numerical techniques. The quantification of the results was carried out using a pseudo two-dimensional, steady state backward implicit finite difference model. A series of studies, interrogating the effects of volumetric flow rate, volume ratio and lead-in length, were carried out to quantitatively investigate hydrodynamic focusing. Chapter 5 details the development and fabrication of patterned photopolymerised and electrochemically polymerised (conducting) monoliths with dimensions in the range of 100-1000μm. The photopolymerised monoliths were characterised using hydrodynamic methods in order to study the flow profile. Electrochemical techniques were used to characterise the conducting monoliths and its composites, using N,N,N’,N’-tetramethyl-p-phenylenediamine. Chapter 6 describes an application of the photopatterned monoliths. A meso-chromatography column was fabricated and immobilised metal affinity chromatography at meso and micro-litre scale was studied inside these columns. Proteins with polyhistidine tags were shown to be successfully separated, purified and quantified under batch and hydrodynamic conditions.","abstract_html":"This thesis describes the design and development of novel micro analytical devices for application in on-line process analytics. The work describes the design, development, numerical simulation and application of these devices for two specific cases: (i) electrochemical detection of bio(chemical) species at micro-scale and (ii) separation and purification of biological reagents using immobilised metal affinity chromatography at micro-litre scale. Chapter 1 provides a general overview and background to the field of process analytics, microreactors and theory related to the mass transfer inside the electrochemical microfluidic devices and meso-chromatography columns. Chapter 2 provides an overview of microfabrication methods and the numerical simulations employed for the development of micro analytical devices used in this thesis. Chapter 3 describes an experimental voltammetric study of enzyme cofactors in batch and hydrodynamic systems and also provides a numerical investigation of mass transfer over electrodes inside microreactors. Chapter 4 investigates the effect of hydrodynamic focusing within a microfluidic device in detail, using experimental and numerical techniques. The quantification of the results was carried out using a pseudo two-dimensional, steady state backward implicit finite difference model. A series of studies, interrogating the effects of volumetric flow rate, volume ratio and lead-in length, were carried out to quantitatively investigate hydrodynamic focusing. Chapter 5 details the development and fabrication of patterned photopolymerised and electrochemically polymerised (conducting) monoliths with dimensions in the range of 100-1000μm. The photopolymerised monoliths were characterised using hydrodynamic methods in order to study the flow profile. Electrochemical techniques were used to characterise the conducting monoliths and its composites, using N,N,N’,N’-tetramethyl-p-phenylenediamine. Chapter 6 describes an application of the photopatterned monoliths. A meso-chromatography column was fabricated and immobilised metal affinity chromatography at meso and micro-litre scale was studied inside these columns. Proteins with polyhistidine tags were shown to be successfully separated, purified and quantified under batch and hydrodynamic conditions.","abstract_has_math":false,"creators":["Deshpande, Abhishek Girish"],"institution":"University of Cambridge","degree_name":"Doctor of Philosophy (PhD)","degree_level":"Doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-11-17","date_published":"2009-11-17","updated_at":"2026-07-22T22:24:31Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/06404a99-7a53-4214-950a-5af8fcfbdcf8/download","https://www.rioxx.net/licenses/all-rights-reserved/"],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.17863/CAM.16075","outbound_label":"DOI","outbound_source":"dc:identifier.doi"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Deshpande, Abhishek Girish"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2009-11-17"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cambridge"]},{"key":"dc:relation.isreferencedby.uri","label":"Dc Relation Isreferencedby URI","values":["https://www.repository.cam.ac.uk/handle/1810/245124"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy (PhD)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/06404a99-7a53-4214-950a-5af8fcfbdcf8/download","https://www.rioxx.net/licenses/all-rights-reserved/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.doi","label":"DOI","values":["10.17863/CAM.16075"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://apollo8-f-pro.lib.cam.ac.uk/bitstreams/ae0fb5a8-0357-437b-b818-d0f34d0d77ec/download"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This thesis describes the design and development of novel micro analytical devices for application in on-line process analytics. 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Chapter 4 investigates the effect of hydrodynamic focusing within a microfluidic device in detail, using experimental and numerical techniques. The quantification of the results was carried out using a pseudo two-dimensional, steady state backward implicit finite difference model. A series of studies, interrogating the effects of volumetric flow rate, volume ratio and lead-in length, were carried out to quantitatively investigate hydrodynamic focusing. Chapter 5 details the development and fabrication of patterned photopolymerised and electrochemically polymerised (conducting) monoliths with dimensions in the range of 100-1000μm. The photopolymerised monoliths were characterised using hydrodynamic methods in order to study the flow profile. Electrochemical techniques were used to characterise the conducting monoliths and its composites, using N,N,N’,N’-tetramethyl-p-phenylenediamine. Chapter 6 describes an application of the photopatterned monoliths. 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