The efficacy of BiOWiSHTM-Thai Aqua, a commercially discontinued microbial product, in remediating oil based drill-cutting mud (DCM) was researched in this study. Experimentation was performed directly on DCM and on sand contaminated with oil extracted from DCM. A gas chromatograph-mass spectrometrer and a respirometer were used for analysis of total petroleum hydrocarbons (TPH) and CO2 production respectively. Five experiments were analyzed by TPH extraction and analysis; four experiments were analyzed by respirometric analysis. The specific microcosm conditions tested in the experiments were control, nutrient-only control, and bioaugmentation product.
This study concluded that there might be potential for bioaugmentation of TPH using BiOWiSHTM-Thai Aqua. However, a more extensive study including multiple replicates of samples over a longer sampling time period is required to make a conclusion. TPH analysis from the Sand Microcosm Experiment suggested that in seven days, the addition of BiOWiSHTM-Thai Aqua improved TPH removal relative to the control by 89% while the nutrient-only control improved by 58%. Respirometric analysis suggested CO2 respiration of glucose overshadowed CO2 respiration from biodegradation. Thus, major conclusions could not be made from the respirometric analysis.
","abstract_html":"<p>The efficacy of BiOWiSHTM-Thai Aqua, a commercially discontinued microbial product, in remediating oil based drill-cutting mud (DCM) was researched in this study. Experimentation was performed directly on DCM and on sand contaminated with oil extracted from DCM. A gas chromatograph-mass spectrometrer and a respirometer were used for analysis of total petroleum hydrocarbons (TPH) and CO2 production respectively. Five experiments were analyzed by TPH extraction and analysis; four experiments were analyzed by respirometric analysis. The specific microcosm conditions tested in the experiments were control, nutrient-only control, and bioaugmentation product.</p> <p>This study concluded that there might be potential for bioaugmentation of TPH using BiOWiSHTM-Thai Aqua. However, a more extensive study including multiple replicates of samples over a longer sampling time period is required to make a conclusion. TPH analysis from the Sand Microcosm Experiment suggested that in seven days, the addition of BiOWiSHTM-Thai Aqua improved TPH removal relative to the control by 89% while the nutrient-only control improved by 58%. Respirometric analysis suggested CO2 respiration of glucose overshadowed CO2 respiration from biodegradation. Thus, major conclusions could not be made from the respirometric analysis.</p>","abstract_has_math":false,"creators":["Zepeda, Diego Jose Cardenas"],"institution":null,"degree_name":"MS in Civil and Environmental Engineering","degree_level":null,"degree_discipline":"Civil and Environmental Engineering","degree_department":null,"school":null,"contributors":["Nirupam Pal"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-06-01T07:00:00Z","date_published":"2015-06-01T07:00:00Z","updated_at":"2026-07-24T01:32:55Z","subjects":["Drill Cutting Mud","Petroleum Hydrocarbons","Bioaugmentation","Bioremediation","Environmental Engineering"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.15368/theses.2015.92"],"render_values":[{"text":"10.15368/theses.2015.92","href":"https://doi.org/10.15368/theses.2015.92","code":true}]}]},"links":{"outbound_url":"https://digitalcommons.calpoly.edu/theses/1440","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nirupam Pal"]},{"key":"dc:creator","label":"Author","values":["Zepeda, Diego Jose Cardenas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2018-06-11T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Civil and Environmental Engineering"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS in Civil and Environmental Engineering"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Drill Cutting Mud","Petroleum Hydrocarbons","Bioaugmentation","Bioremediation","Environmental Engineering"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.calpoly.edu/theses/1440","10.15368/theses.2015.92"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The efficacy of BiOWiSHTM-Thai Aqua, a commercially discontinued microbial product, in remediating oil based drill-cutting mud (DCM) was researched in this study. Experimentation was performed directly on DCM and on sand contaminated with oil extracted from DCM. A gas chromatograph-mass spectrometrer and a respirometer were used for analysis of total petroleum hydrocarbons (TPH) and CO2 production respectively. Five experiments were analyzed by TPH extraction and analysis; four experiments were analyzed by respirometric analysis. The specific microcosm conditions tested in the experiments were control, nutrient-only control, and bioaugmentation product.
This study concluded that there might be potential for bioaugmentation of TPH using BiOWiSHTM-Thai Aqua. However, a more extensive study including multiple replicates of samples over a longer sampling time period is required to make a conclusion. TPH analysis from the Sand Microcosm Experiment suggested that in seven days, the addition of BiOWiSHTM-Thai Aqua improved TPH removal relative to the control by 89% while the nutrient-only control improved by 58%. Respirometric analysis suggested CO2 respiration of glucose overshadowed CO2 respiration from biodegradation. Thus, major conclusions could not be made from the respirometric analysis.
"]},{"key":"dc:title","label":"Title","values":["Treatment of Petroleum Hydrocarbons in Oil-Based Drill Cutting Mud Using BiOWiSH Bioaugmentation Products"]}]}],"canonical_facts":{"dc:contributor":["Nirupam Pal"],"dc:creator":["Zepeda, Diego Jose Cardenas"],"dc:date.available":["2018-06-11T07:00:00Z"],"dc:description.abstract":["The efficacy of BiOWiSHTM-Thai Aqua, a commercially discontinued microbial product, in remediating oil based drill-cutting mud (DCM) was researched in this study. Experimentation was performed directly on DCM and on sand contaminated with oil extracted from DCM. A gas chromatograph-mass spectrometrer and a respirometer were used for analysis of total petroleum hydrocarbons (TPH) and CO2 production respectively. Five experiments were analyzed by TPH extraction and analysis; four experiments were analyzed by respirometric analysis. The specific microcosm conditions tested in the experiments were control, nutrient-only control, and bioaugmentation product.
This study concluded that there might be potential for bioaugmentation of TPH using BiOWiSHTM-Thai Aqua. However, a more extensive study including multiple replicates of samples over a longer sampling time period is required to make a conclusion. TPH analysis from the Sand Microcosm Experiment suggested that in seven days, the addition of BiOWiSHTM-Thai Aqua improved TPH removal relative to the control by 89% while the nutrient-only control improved by 58%. Respirometric analysis suggested CO2 respiration of glucose overshadowed CO2 respiration from biodegradation. Thus, major conclusions could not be made from the respirometric analysis.
"],"dc:identifier":["https://digitalcommons.calpoly.edu/theses/1440","10.15368/theses.2015.92"],"dc:subject":["Drill Cutting Mud","Petroleum Hydrocarbons","Bioaugmentation","Bioremediation","Environmental Engineering"],"dc:title":["Treatment of Petroleum Hydrocarbons in Oil-Based Drill Cutting Mud Using BiOWiSH Bioaugmentation Products"],"thesis:degree_discipline":["Civil and Environmental Engineering"],"thesis:degree_name":["MS in Civil and Environmental Engineering"]},"updated_at":"2026-07-24T01:32:55Z"}