This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for quality and quantity. The project is divided into improving three aspects of the process: 1) plasmid identification, 2) plasmid purity evaluation, and 3) process yield. Plasmid identification is now simpler, more robust and has zero ambiguity. Plasmid purity evaluation is now measured with computer software, which reduces user error and eliminates subjectivity. Using the nascent metrics provided by the improved identification and purity evaluation techniques, process yield was analyzed and improved. The hypotheses on yield improvement and the information gleaned from their resulting experiments provide a foundation for further process improvement.
","abstract_html":"<p>This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for quality and quantity. The project is divided into improving three aspects of the process: 1) plasmid identification, 2) plasmid purity evaluation, and 3) process yield. Plasmid identification is now simpler, more robust and has zero ambiguity. Plasmid purity evaluation is now measured with computer software, which reduces user error and eliminates subjectivity. Using the nascent metrics provided by the improved identification and purity evaluation techniques, process yield was analyzed and improved. The hypotheses on yield improvement and the information gleaned from their resulting experiments provide a foundation for further process improvement.</p>","abstract_has_math":false,"creators":["Shepherd, Trevor J"],"institution":null,"degree_name":"MS in Biomedical Engineering","degree_level":null,"degree_discipline":"Biomedical and General Engineering","degree_department":null,"school":null,"contributors":["Lanny Griffin"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-06-01T07:00:00Z","date_published":"2013-06-01T07:00:00Z","updated_at":"2026-07-24T01:31:17Z","subjects":["DNA","Plasmid","Transformation","Purification","Identification","Process","Manufacturing","Biotechnology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.15368/theses.2013.57"],"render_values":[{"text":"10.15368/theses.2013.57","href":"https://doi.org/10.15368/theses.2013.57","code":true}]}]},"links":{"outbound_url":"https://digitalcommons.calpoly.edu/theses/1016","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lanny Griffin"]},{"key":"dc:creator","label":"Author","values":["Shepherd, Trevor J"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-08-14T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical and General Engineering"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS in Biomedical Engineering"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DNA","Plasmid","Transformation","Purification","Identification","Process","Manufacturing","Biotechnology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.calpoly.edu/theses/1016","10.15368/theses.2013.57"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for quality and quantity. The project is divided into improving three aspects of the process: 1) plasmid identification, 2) plasmid purity evaluation, and 3) process yield. Plasmid identification is now simpler, more robust and has zero ambiguity. Plasmid purity evaluation is now measured with computer software, which reduces user error and eliminates subjectivity. Using the nascent metrics provided by the improved identification and purity evaluation techniques, process yield was analyzed and improved. The hypotheses on yield improvement and the information gleaned from their resulting experiments provide a foundation for further process improvement.
"]},{"key":"dc:title","label":"Title","values":["DNA Purification Process Optimization at Life Technologies Corporation"]}]}],"canonical_facts":{"dc:contributor":["Lanny Griffin"],"dc:creator":["Shepherd, Trevor J"],"dc:date.available":["2013-08-14T07:00:00Z"],"dc:description.abstract":["This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for quality and quantity. The project is divided into improving three aspects of the process: 1) plasmid identification, 2) plasmid purity evaluation, and 3) process yield. Plasmid identification is now simpler, more robust and has zero ambiguity. Plasmid purity evaluation is now measured with computer software, which reduces user error and eliminates subjectivity. Using the nascent metrics provided by the improved identification and purity evaluation techniques, process yield was analyzed and improved. The hypotheses on yield improvement and the information gleaned from their resulting experiments provide a foundation for further process improvement.
"],"dc:identifier":["https://digitalcommons.calpoly.edu/theses/1016","10.15368/theses.2013.57"],"dc:subject":["DNA","Plasmid","Transformation","Purification","Identification","Process","Manufacturing","Biotechnology"],"dc:title":["DNA Purification Process Optimization at Life Technologies Corporation"],"thesis:degree_discipline":["Biomedical and General Engineering"],"thesis:degree_name":["MS in Biomedical Engineering"]},"updated_at":"2026-07-24T01:31:17Z"}