While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands of different species in order to better understand applications in human health and the dairy industry. In this study, we examined global protein expression during two states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in abundance in the two experiments, we observed many similarities in proteins related to structural dynamics and mRNA processing within these two mammary epithelial cell types. Intriguingly, we observed several differences in the regulation of metabolic proteins, highlighting the distinct pathways by which different species probably metabolize energy and synthesize milk components.
","abstract_html":"<p>While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands of different species in order to better understand applications in human health and the dairy industry. In this study, we examined global protein expression during two states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in abundance in the two experiments, we observed many similarities in proteins related to structural dynamics and mRNA processing within these two mammary epithelial cell types. Intriguingly, we observed several differences in the regulation of metabolic proteins, highlighting the distinct pathways by which different species probably metabolize energy and synthesize milk components.</p>","abstract_has_math":false,"creators":["Strand, Laura Therese"],"institution":null,"degree_name":"MS in Agriculture - Animal Science","degree_level":null,"degree_discipline":"Animal Science","degree_department":null,"school":null,"contributors":["Daniel Gunnar Peterson"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-06-01T07:00:00Z","date_published":"2012-06-01T07:00:00Z","updated_at":"2026-07-24T01:31:17Z","subjects":["Mammary","Mammary Development","Developmental Biology","Cellular Differentiation","Epithelial Differentiation","Molecular Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.15368/theses.2012.141"],"render_values":[{"text":"10.15368/theses.2012.141","href":"https://doi.org/10.15368/theses.2012.141","code":true}]}]},"links":{"outbound_url":"https://digitalcommons.calpoly.edu/theses/825","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Daniel Gunnar Peterson"]},{"key":"dc:creator","label":"Author","values":["Strand, Laura Therese"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2012-07-10T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Science"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS in Agriculture - Animal Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Mammary","Mammary Development","Developmental Biology","Cellular Differentiation","Epithelial Differentiation","Molecular Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.calpoly.edu/theses/825","10.15368/theses.2012.141"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands of different species in order to better understand applications in human health and the dairy industry. In this study, we examined global protein expression during two states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in abundance in the two experiments, we observed many similarities in proteins related to structural dynamics and mRNA processing within these two mammary epithelial cell types. Intriguingly, we observed several differences in the regulation of metabolic proteins, highlighting the distinct pathways by which different species probably metabolize energy and synthesize milk components.
"]},{"key":"dc:title","label":"Title","values":["A Proteomic Analysis of Differentiation in the Mammary Epithelium"]}]}],"canonical_facts":{"dc:contributor":["Daniel Gunnar Peterson"],"dc:creator":["Strand, Laura Therese"],"dc:date.available":["2012-07-10T07:00:00Z"],"dc:description.abstract":["While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands of different species in order to better understand applications in human health and the dairy industry. In this study, we examined global protein expression during two states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and primary mammary epithelial cells isolated from pregnant and lactating mice. When comparing the lists of proteins that differed in abundance in the two experiments, we observed many similarities in proteins related to structural dynamics and mRNA processing within these two mammary epithelial cell types. Intriguingly, we observed several differences in the regulation of metabolic proteins, highlighting the distinct pathways by which different species probably metabolize energy and synthesize milk components.
"],"dc:identifier":["https://digitalcommons.calpoly.edu/theses/825","10.15368/theses.2012.141"],"dc:subject":["Mammary","Mammary Development","Developmental Biology","Cellular Differentiation","Epithelial Differentiation","Molecular Biology"],"dc:title":["A Proteomic Analysis of Differentiation in the Mammary Epithelium"],"thesis:degree_discipline":["Animal Science"],"thesis:degree_name":["MS in Agriculture - Animal Science"]},"updated_at":"2026-07-24T01:31:17Z"}